基于1H NMR代谢组学技术的传统产区与种植基地太子参化学成分分析  

Analysis of Chemical Compositions in Pseudostellariae Radix from Traditional Field and Cultivated Fields by 1H NMR-based Metabolomics

作　　者：华愉教 侯娅[1] 王胜男[1] 刘训红[1] 邹立思[1] 谷巍[1] 罗益远 刘娟秀[1] 

机构地区：[1]南京中医药大学,南京210023

出　　处：《天然产物研究与开发》2016年第10期1510-1516,共7页Natural Product Research and Development

基　　金：国家自然科学基金项目(81274016;81473312);江苏高校优势学科建设工程资助项目(ysxk-2014);江苏高校品牌专业建设工程资助项目(PPZY2015A070)

摘　　要：探讨不同产区太子参代谢物合成积累的差异。采用1H NMR代谢组学技术结合多元统计分析对传统产区与种植基地太子参中化学成分进行比较分析,并找出差异成分。共指认出35个化学成分;多元统计分析显示,传统产区与种植基地太子参样品能明显分开,其中差异显著的化学成分有12个,精氨酸、谷氨酸、乙酰乙酸、琥珀酸的相对含量在传统产区太子参中较高;赖氨酸、二甲基甘氨酸钠、苯丙氨酸、蔗糖、木糖、槲皮素、富马酸、阿魏酸的相对含量在种植基地太子参中较高。本研究结果为揭示不同产区太子参代谢物合成积累的变化规律及其药材的品质形成机制提供基础资料。To investigate the differences in accumulation and biosynthesis of metabolites in Pseudostellariae Radix. 1 H NMR based metabolomic approach combined with muhivariate statistical analysis was used to investigate the chemical compositions and find the different metabolites in Pseudostellariae Radix from traditional field and cultivated fields. 35 Metabdites were identified in the 1 H NMR spectrum, and 12 of which showed remarkable differences. The multivariate statistical analysis showed that Pseudostellariae Radix from cultivated fields and from traditional field can be separated obviously. The levels of arginine, glutamate, acetoacetate and succinate in Pseudostellariae Radix from traditional field were higher, while the cultivated Pseudostellariae Radix contained more lysine, dimethylglycine, phenylalanine, sucrose, xylose, quercetin, fumarate and ferulic acid. This study will provide the basic information for exploring the quality formation mechanism and revealing the differences in accumulation and biosynthesis of metabolites in Pseudostellariae Radix.

关 键 词：太子参 传统产区与种植基地 化学成分 1H NMR 多元统计分析 

分 类 号：R917[医药卫生—药物分析学]