弯柄灵芝总三萜加速溶剂提取工艺及其清除DPPH自由基活性研究  

Accelerated Solvent Extraction and DPPH Free Radical Scavenging Activity of Triterpenoids from Ganoderma flexipes

作　　者：陈德力[1] 王小兵[2] 吕伟 贾立伟 冯剑[1] 郑希龙[1] 

机构地区：[1]中国医学科学院&北京协和医学院药用植物研究所海南分所海南省南药资源保护与开发重点实验室,海口570311 [2]海南大学材料与化工学院,海口570228 [3]北京三联国际投资有限责任公司,北京100193

出　　处：《天然产物研究与开发》2016年第10期1597-1601,1617,共6页Natural Product Research and Development

基　　金：公益性行业(农业)科研专项(201303117);海南省中药现代化专项(2015ZY25)

摘　　要：采用加速溶剂萃取法(ASE),进行单因素实验和L9(33)正交实验,确定弯柄灵芝总三萜最佳提取工艺,结合DPPH法对弯柄灵芝总三萜清除DPPH自由基活性评价。结果表明,ASE最佳提取工艺条件为60%甲醇(氯仿-甲醇)为提取溶剂,提取温度180℃,提取时间12 min,静态循环提取次数2次,其中提取温度对总三萜提取率具有极显著性影响。在最优条件下,弯柄灵芝总三萜提取率为(2.14±0.18)%。体外DPPH清除实验结果显示,ASE最优提取工艺及传统方法提取所得总三萜对DPPH自由基的IC50值分别为129.5和273.4μg/m L。该工艺与传统加热回流提取方法相比,具有操作简便、缩短提取时间、节能耗、三萜得率高、清除DPPH自由基活性强等优点,可用于弯柄灵芝总三萜的提取生产,为弯柄灵芝产品的开发提供了有益的参考。In this study, accelerated solvent extraction （ASE） method was used to extract triterpenoids from Ganoderma flexipes. Single factor and L9 （3 3） orthogonal experiments was applied to optimize the extraction conditions. In addition, free radical scavenging activities of the extracts were evaluated by DPPH assays. The results showed that the optimal extraction conditions were 60% methanol （chloroform and methanol） as the extraction solvent, extraction temperature of 180℃, extraction time of 12 min and with 2 times of static extraction, in which extraction temperature had extremely significant effect on the extraction yield of triterpenoids. Under the optimized conditions, the extraction yield of total triterpenoids from G. flexipes was up to （2.14 ± 0. 18 ）%. In the DPPH exp＇eriments, the IC3o value of total triterpenoids obtained from the optimal ASE extraction process and the traditional methods were 129.5 and 273.4 μg/mL,respectively. Comparing with the traditional heat reflux extraction, ASE was simple, rapid, economical, practical, which can be used for the extraction of triterpenoids from G.flexipes.

关 键 词：弯柄灵芝 三萜 加速溶剂萃取 提取工艺 DPPH 

分 类 号：R284.2[医药卫生—中药学]