资木瓜总黄酮的体内外抗氧化活性研究  

In vitro and in vivo Antioxidant Properties of Total Flavonoids from Chaenomeles speciosa

作　　者：张津鸣 钟筱婷 盛德乔[1] 喻玲玲[1] 李世刚[1] 柳蔚[1] 

机构地区：[1]三峡大学医学院,宜昌443002

出　　处：《天然产物研究与开发》2016年第10期1602-1606,共5页Natural Product Research and Development

基　　金：国家自然科学基金面上项目(81274166);湖北省教育厅科学研究项目(B2013177);湖北省宜昌市科学研究与开发项目(A13301-58)

摘　　要：采用70%乙醇回流提取,大孔吸附树脂纯化得到资木瓜总黄酮,进一步研究其体内外抗氧化活性。检测资木瓜对Fe3+的还原力,分析资木瓜对羟自由基、DPPH·自由基以及超氧阴离子自由基的清除能力,检测对LPS诱导的氧化应激小鼠模型血液中SOD和MDA的影响。结果发现资木瓜总黄酮具有良好的还原能力,对·OH和DPPH·自由基的最大清除率分别是97.8%、69.8%,但对超氧阴离子的清除率较低仅为38.3%;在体内实验中资木瓜能增加小鼠血清中SOD活性、降低MDA浓度。说明资木瓜总黄酮通过直接清除自由基、增加机体抗氧化酶SOD的活性、减少脂质过氧化物的生成,产生抗氧化活性。Total flavonoids of Chaenomeles speciosa （TFCS） were purified with macroporous resin from the 70% ethanol reflux extract, and its antioxidant activities were then assayed. The in vitro antioxidant results of TFCS showed that the efficacy on reducing power, hydroxyl and 1,1-diphenyl-2-picrylhydrazyl （DPPH） were 99.99% ,97.8%, and 69.8%, respectively,and the efficacy on superoxide anion was merely 38.3%. The in vivo test showed that TFCS significantly increased the superoxide dismutase （SOD） activity, whereas an reduced level of malondialdehyde （MDA） products was obtained in lipopolysaccharide （LPS） induced oxidative stress mice model. Results suggested that TFCS displayed a strong antioxidant activity by direct scavenging of free radicals, increasing the main antioxidant enzyme SOD activity, but reduced the formation of lipid peroxidation MDA products.

关 键 词：木瓜 黄酮 抗氧化 体内外 

分 类 号：R285.5[医药卫生—中药学]