益母草注射液水溶性生物碱--胆碱和胡芦巴碱对子宫收缩活动的影响  

Water soluble alkaloid of Motherwort injection——effects of choline and acetylcholine on uterine contraction activity

作　　者：代良萍 谢晓芳[1] 熊亮[1] 任波[1] 胡婷婷[1] 董艳红[1] 彭成[1] 

机构地区：[1]成都中医药大学药学院,中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都610075

出　　处：《时珍国医国药》2016年第10期2328-2331,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81303209);中药药理四川省青年基金科技创新研究团队(No.2014TD0007);中药资源四川省青年科技创新研究团队(No.2015TD0028)

摘　　要：目的探讨益母草注射液水溶性生物碱单体胆碱、葫芦巴碱对正常大鼠、豚鼠离体、正常豚鼠在体子宫活动的影响。方法从益母草注射液水溶性总生物碱部位中提取分离出单体胆碱、葫芦巴碱；采用正常（大鼠、豚鼠）离体子宫模型、未孕豚鼠豚鼠在体子宫模型观察其对子宫收缩的活动力、张力和频率的影响，用BL-420E生物机能系统记录子宫收缩。结果离体子宫试验表明，胆碱、葫芦巴碱3×10^-5可显著降低大鼠正常离体子宫收缩最大值（P〈0．05），显著增加豚鼠正常离体子宫活动力（P〈0．05），显著降低子宫收缩频率（P〈0．05）；在体子宫试验表明，肌肉注射1mg／kg胆碱，能够显著降低给药后60～80min正常豚鼠子宫的收缩频率（P〈0．05），显著增加给药后0～10min子宫收缩张力最大值（P〈0．05或P〈0．01），对子宫收缩活力，收缩张力均值及最小值无明显影响；肌肉注射1mg／kg葫芦巴碱，能够显著增加给药后20～40min正常豚鼠子宫的收缩活力（P〈0．05），显著增加给药后20～90min子宫收缩张力最大值（P〈0．05或P〈0．01），对子宫收缩频率、收缩张力均值及最小值无明显影响。结论胆碱、葫芦巴碱具有兴奋正常豚鼠离体子宫和在体子宫平滑肌活动的作用。Objective To study the effects of Leonurus Heterophyllus Injection Water Soluble alkaloid monomer choline, fenu- greek trigonelline on normal rats and guinea pigs from the body and normal guinea pigs in vivo uterine activity. Methods Mother- won injection of water - soluble extract from total alkaloids isolated parts monomer choline, trigonelline ; use normal （ rat, guinea pig） isolated uterus model, nonpregnant guinea guinea pig model was observed in uterine contractions of the uterus the impact of motility, tension and frequency, with the BL- 420E biological function system records uterine contractions. Results In vitro ex- periments showed that the uterus, choline, trigonelline 3 × 10^-2 can significantly reduce the normal rat uterine contractions maxi- mum value （P 〈 0. 05 ） , a significant increase in guinea pig normal uterine motility （P 〈 0.05 ） significantly reduced the fre- quency of uterine contractions （P 〈 0. 05） ; In the body of the uterus tests showed that intramuscular injection of 1 mg / kg cho- line, can significantly reduce 60 - 80rain normal guinea pig after administration of uterine contraction frequency （ P 〈 0. 05 ）, a significant increase in uterine contractions after administration 0 - lOmin maximum tension （ P 〈 0.05 or P 〈 0.01 ）, the vitality of uterine contractions, mean and mininmm shrinkage tension no significant effect; Intramuscular injection of lmg / kg trigonel- line, can significantly increase after administration 20 - 40min normal uterine contractions in guinea pigs vitality （ P 〈 0.05 ）, a significant increase in uterine contractions 20 - 90min after administration of the maximum tension （ P 〈 0.05 or P 〈 0.01 ）, the frequency of uterine contractions, mean and minimum shrinkage tension had no significant effect. Conclusion Choline, trigonel- line has an activity in exciting excited the normal role of guinea pig uterus and uterine smooth muscle.

关 键 词：水溶性总生物碱 胆碱 葫芦巴碱 益母草注射液 缩宫 离体子宫 在体子宫 

分 类 号：R285.5[医药卫生—中药学]