基于β-防御素-2的中药骨碎补对气管切开留置气管套大鼠肺部免疫功能的干预作用  

The effect of Rhizoma Drynariae on pulmonary immunity based on β-defensin-2 in rats after tracheotomy

作　　者：张冉令 潘宇政[2] 黄文川[2] 彭玲玲[2] 

机构地区：[1]四川省内江市中医院,641000 [2]广西医科大学第一附属医院,广西南宁530021

出　　处：《时珍国医国药》2016年第10期2365-2368,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(NSFC.81460687);广西壮族自治区卫生厅中医药科技专项(GZPT13-36)

摘　　要：目的探讨气管切开插管留置大鼠肺组织中β-防御素-2 mRNA(r BD2)表达及T细胞亚群(CD3、CD4、CD8)含量和肺泡灌洗液s Ig A含量变化规律及骨碎补对其的干预作用。方法将72只SPF级健康雄性Sprague-Dawley(SD)大鼠分成正常对照组(A)、气管切开插管留置模型非用药组(B)及模型用药组(C),每组各24只。制备大鼠气管切开插管留置模型。在给药后第24h、72h、168h三个时间段活体取材。采集肺泡灌洗液及肺组织,检测肺组织β-防御素-2(r BD2)mRNA表达、T细胞亚群(CD3、CD4、CD8)以及肺泡灌洗液s Ig A含量。结果 B组肺组织r BD2 mRNA表达在造模后第24小时明显升高,在第72小时开始下降,至第168小时降到最低值;用中药骨碎补干预后,r BD2表达开始明显升高,至第72小时表达上调到最高值,在第168小时表达则稍有下降。气切插管后大鼠肺泡灌洗液s Ig A含量在第24小时和第72小时明显高于正常大鼠,但在第168小时则明显回落。用中药骨碎补干预后,气切插管大鼠肺泡灌洗液s Ig A含量在第24小时即明显上升,在第168小时亦没有下降。气管切开后肺组织CD3含量明显降低,在168小时达到最低值;服用骨碎补后CD3含量出现上升,在72小时达到最大值,在168小时时则稍有回落。气管切开插管后肺组织CD4含量出现短暂上升,在24小时达到最大值,而后则明显下降,其下降在第72小时达到最低值,在168小时则稍有上升,服用骨碎补后在第72小时时明显上升,在第168小时上升达到最大值。气管切开后肺组织CD8含量明显上升,在第72小时上升达到最大值,而后168小时则明显回落;服用骨碎补后在第72小时开始下降,到168小时其下降达到最低值。结论骨碎补能够上调气切插管大鼠肺组织β-防御素-2 mRNA的表达,提高肺泡灌洗液s Ig A含量和改善肺组织T细胞亚群失衡的作用。Objective To explore the beta defense - 2 mRNA expression and T cell subsets content in the lung tissue of rats af- ter tracheotomy , sIgA content change rule in inalveolar lavage and Rhizoma Drynariae intervention effect on it. Methods All of 72 SPF health male SD rats were divided into three groups： normal control group （A）, model with Tracheotomy tube （B）and the treatment group（C）. To prepare model with tracheotomy. Collecting specimen at 24h ,72h, 168h three phases after the success of the model. Collection of lung tissue and alveolar lavage fluid. Detection of beta defense - 2 mRNA expression and content of T cell subsets in lung tissue and the sIgA level in alveolar lavages. Results （ 1 ） The BD2 mRNA expression in the lung tissue was signif- icantly increased at the 24h after tracheotomy, it starts to fall in 72h and decline to the lowest value inl68h;With Chinese medi- cine Rhizoma Drynariae intervention, The rBD2 mRNA expression was obviously increased and up to the highest value at 72h , but the expression was slightly decreased in 168h. （2）The slgA content of rat in alveolar lavagc fluid was increased in 24h and 72h two hours after tracheotomy ,but it decreased at 168h. With Chinese medicine Rhizoma Drynariae intervention,the sIgA con- tent was increased in 24h and did not fall at 168h. （3）The content of CD3 in lung tissue was significantly decreased after tracheot- omy ,it reached the lowest value in 168h ;with taking Rhizoma Drynariae,the content of CD3 appears to rise, reaching the maxi- mum value in 72h, and it is slightly down at 168h. （4） The content of CD4 in lung tissue was transient increased and then de- creased significantly after tracheotomy, it declined the lowest value in the 72h and slightly increased in 168h ;with taking Rhizoma Drynariae ,it starts to increase at 72h and reached the maximum value at 168h. （5）The CD8 levels in lung tissue increased sig- nificantly after tracheotomy, it reached the maximum at the 72h ; with taking Rhizoma Drynariae, it b

关 键 词：骨碎补 气管切开插管 β防御素2 SIGA T细胞亚群 

分 类 号：R285.5[医药卫生—中药学]