南瓜叶多糖纯化工艺研究  

Purification Process of Polysaccharide from Pumpkin Leaves

作　　者：张强[1] 

机构地区：[1]西北农林科技大学林学院,陕西杨凌712100

出　　处：《广州化工》2016年第21期37-39,共3页GuangZhou Chemical Industry

基　　金：国家林业局林业公益性行业专项"林源食用叶类深加工与产品质量控制技术研究"(201304811)

摘　　要：南瓜叶多糖含量较高,为了开发利用南瓜叶多糖资源,利用大孔树脂纯化南瓜叶粗多糖,研究了多糖溶液浓度,温度,p H对树脂吸附率的影响,以及大孔树脂加入量,温度,时间对脱色率的影响。结果发现,大孔树脂AB-8和D101对南瓜叶多糖的吸附能力均很低,调节南瓜叶多糖浓度、温度和p H不能显著增加使南瓜叶多糖的吸附率;大孔树脂D101对南瓜叶多糖脱色效果较好,最佳工艺条件为,添加量为南瓜叶多糖溶液体积的5%,脱色时间为3 h,温度40℃。脱色后,多糖含量由32.14%提高到58.68%,纯度提高了82.6%,而蛋白质含量由13.93%降低到4.59%。大孔树脂可有效提纯南瓜叶多糖。Pumpkin （Cucurbita moschata Duch. ） is a popular food material in China, however, pumpkin leaves have not yet been industrial utilized although they are rich in protein, trace elements, carotenes, polyphenols and other nutrition or function components. In order to utilize the pumpkin leaf polysaccharide resources, the macroporous resin was used to purify the polysaccharide. The effects of polysaecharide concentration, temperature, pH on the resin absorbing rate, and the effects of resin mass, time, temperature on the pumpkin leaf polysaccharide decoration were studied. The results showed that the resin AB-8 and D101 could not absorb the pumpkin leaf polysaccharide efficiently. The optimum extraction condition was as follows ： resin mass of 5 % of the volume of polysaccharide solution, 30 min and temperature at 40℃. Under this condition, the purity of pumpkin leaf polysaccharide increased from 32. 14% to 58.68% , and the content of protein was decreased from 13.93% to 4. 59%.

关 键 词：南瓜叶 多糖 大孔树脂 脱色 

分 类 号：S713[农业科学—林学]