HPLC法测定红景天原液中红景天苷的含量  

Determination of the Content of Salidroside in Rhodiola Liquid by HPLC

作　　者：李笑[1] 吴秀专 褚燕斌 蒋瑶[1] 王雪慧[1] 马宁[1] 

机构地区：[1]长沙医学院药学院,湖南长沙410219

出　　处：《香料香精化妆品》2016年第5期50-53,共4页Flavour Fragrance Cosmetics

基　　金：2015年度国家级大学生研究性学习和创新性实验计划项目(项目编号201510823002);长沙医学院2016年度校级立项大学生研究性学习和创新性实验计划项目[项目编号:长医教〔2016〕19号]

摘　　要：建立了红景天原液中红景天苷的含量测定方法,为含红景天化妆品的质量控制提供方法和依据。具体采用反相高效液相色谱法,色谱柱为ODS-2HYPERSIL（150 mm×4.6 mm×5μm）,流动相为甲醇-水（体积比12∶88）,流速为1.0 m L/min,检测波长为275 nm,柱温为30℃。结果发现,红景天苷进样量在0.0122~0.0612μg范围内与峰面积积分值呈现良好线性关系（r=0.9993）;精密度、稳定性、重复性试验的相对标准偏差（RSD）〈2%;平均加样回收率为99.52%,RSD为1.43%（n=9）。该方法准确、稳定、重复性好,可用于含红景天化妆品的质量控制。To establish a method for determination of salidroside in rhodiola liquid, provide the basis for setting quality standards, HPLC method was adopted. The determination was performed on ODS - 2HYPERSIL （150 mm × 4.6 mm × 5 lam） column with mobile phase of methanol - water （12/88, volume ratio） at the flow rate of 1.0 mL/min. The detection wavelength was 275 nm and the column temperature was maintained at 30 ℃. The results showed that the linear range of salidroside were in the range of 0.0122 to 0.0612 μg （r = 0.9993）. RSDs of precision, stability, and reproducibility tests were all lower than 2%. Average recovery rate was 99.52% （ RSD=1.43%, n =9）. The method is accurate, reliable and reproducible, and it can be used for quality control of cosmetics containing rhodiola.

关 键 词：化妆品 红景天 红景天苷 含量测定 高效液相色谱法 

分 类 号：TQ658[化学工程—精细化工]