从日本进境邮寄物中截获伤残短体线虫  

Root-lesion nematode,Pratylenchus vulnus,on imported mails from Japan

作　　者：闫世艳[1,2] 陈艳[1] 边勇[3] 高文娜[3] 简恒[2] 李倞平 张继军[3] 

机构地区：[1]北京农学院,北京102206 [2]中国农业大学,北京100193 [3]北京出入境检验检疫局,北京100026

出　　处：《生物安全学报》2016年第4期291-294,共4页Journal of biosafety

基　　金：北京市教委2015年度科研计划项目(KM201510020004);国家质检总局科技计划项目(2015IK023)

摘　　要：【背景】2015年北京出入境检验检疫技术中心植物实验室从日本邮寄进境的鸡爪槭中检出一种短体线虫。【方法】通过采用形态学鉴定、rDNA序列测定及系统进化分析,对该线虫进行鉴定。【结果】将该线虫的形态特征和测计值与已知种群比较,与Pratylenchus vulnus最为接近;系统进化分析表明,其r DNA-ITS区序列特征与P.vulnus序列相似度为99%;系统发育树表明,其与P.vulnus聚为一组,证实该线虫为伤残短体线虫P.vulnus。【结论与意义】伤残短体线虫是一种重要的植物寄生线虫,我国进出境检疫部门曾多次从入境货物中检出该线虫,但从入境邮寄物中检中尚属首次。【 Background】 In 2015,one Pratylenchus population was collected in the roots and growdng media of Acer palmatum im-ported from Japan by plant quarantine lab of Beijing entry-exit inspection and quarantine bureau, China.【 Method】 In the study the population was identified by morphological measurements, rDNA sequence and phylogenetic analys is.【 Result】 Comparing the mor-phological characteristics and measurements with know,n species, the nematode is phylogenetically close to Pratylenchus vulnus , ac- croding to the sequence of rDNA-ITS region with 99%. The results confirmed that this population was P. vunus . 【 Conclusion and significance】 P. vulnus is an important plant parasitic nematodes that has been intercepted from the entry of goods many times by our entry-exit inspection and quarantine departments, but it is the first time in the mail quarantine.

关 键 词：邮寄物检疫 伤残短体线虫 鸡爪槭 RDNA-ITS 系统发育 鉴定 

分 类 号：S41-3[农业科学—植物保护]