GIPR在棕色脂肪细胞分化中的表达及功能分析  

Expression and function of GIP receptor in brown adipocytes

作　　者：李广森[1] 戴婧 李吉[1] 蔡莉 柯孟婷 李杨[1] 孙家忠[1] 刘超[4] 

机构地区：[1]郧阳区人民医院太和医院郧阳分院内分泌科 [2]武汉市第五人民医院 [3]武汉市中山医院 [4]湖北科技学院糖尿病与心脑血管病变湖北省重点实验室

出　　处：《中华内分泌代谢杂志》2016年第10期857-861,共5页Chinese Journal of Endocrinology and Metabolism

摘　　要：采用葡萄糖依赖性胰岛素释放多肽（ GIP）干预诱导分化成熟的棕色脂肪细胞,以Western印迹分析GIP受体（GIPR）、解耦联蛋白1（UCP-1）、cAMP反应元件结合蛋白（CREB）等的蛋白表达,ELISA检测细胞内cAMP浓度变化。结果显示,分离培养的大鼠脂肪细胞光镜下呈典型棕色脂肪细胞形态,油红O染色可见细胞内大量脂滴聚集；免疫组化检测可见细胞内UCP-1表达；在棕色脂肪细胞分化后期GIPR mRNA表达量呈上升趋势,免疫组化检测可见棕色脂肪细胞内GIPR蛋白表达。1和100 nmol/L的GIP显著升高棕色脂肪细胞GIPR、UCP-1和CREB的蛋白表达,10 nmol/L时明显降低；各种浓度GIP干预24 h后棕色脂肪细胞内cAMP浓度均显著升高（P〈0.05）。结果提示GIPR可能参与棕色脂肪细胞的分化。[Summary] After differentiated primary brown fat cells were incubated with glucose-dependent insulinotropic polypeptide（GIP）, GIP receptor（GIPR）, uncoupling protein 1（UCP-1）, and CREB protein expressions were measured by Western blot, and intracellular cAMP was detected by ELISA. The results showed that cultured primary adipocytes manifested a typical morphology of brown fat cells, with a large amount of lipid droplet accumulation shown by oil red O and UCP-1 expression detected by immunohistochemistry. On the later stage of brown adipocyte differentiation, GIPR mRNA expression revealed an increased tendency. GIPR protein expression was detected by immunohistochemistry in brown fat cells. 1 and 100 nmol/L GIP significantly increased GIPR,UCP-1, and CREB protein expressions while 10 nmol/L GIP decreased the expressions of these genes. Treatment with various concentrations of GIP for 24 hours raised cAMP concentrations in brown fat cells. These results suggest that GIPR plays a critical role in the differentiation course of primary brown adipocyte.

关 键 词：糖尿病 2型 棕色脂肪细胞 葡萄糖依耐性胰岛素释放多肽受体 DIABETES mellitus type 2 

分 类 号：R589.2[医药卫生—内分泌]