亚低温与氨磷汀对辐照后小鼠辐射防护作用的比较  

Radioprotective effect of mild hypothermia versus amifostine on acute radiation injury in mice

作　　者：王新钢[1] 李曙芳[1] 王永丽[1] 黄立群[1] 岳娟[1] 安全[1] 闻建华[1] 

机构地区：[1]中国辐射防护研究院放射医学与环境医学研究所,太原医学硕士030006

出　　处：《医学研究生学报》2016年第11期1186-1190,共5页Journal of Medical Postgraduates

基　　金：山西省实验动物专项资金项目[2013(1007)]

摘　　要：目的氨磷汀作为一种泛细胞辐射防护剂已在临床放化疗展开应用,但其不良反应较多。文中拟比较亚低温处理与氨磷汀处理对辐射损伤小鼠辐射防护作用的效果。方法 175只雄性BALB/C小鼠按随机数字表法分为正常对照组、单纯亚低温组、单纯照射组、氨磷汀组和亚低温组,每组35只。单纯照射组、亚低温组、氨磷汀组小鼠均接受6Gy60Coγ射线单次全身辐照,亚低温组在照后即刻进行亚低温干预并维持6 h,单纯亚低温组在假辐照后给予亚低温处理并维持6 h,氨磷汀组小鼠接受辐照前0.5 h腹腔注射氨磷汀,观察骨髓病理组织变化,检测辐照后1、3、7、14、21、28 d小鼠骨髓有核细胞数,辐照后6 h和24 h小鼠血清丙二醛含量、谷胱甘肽过氧化物酶(glutathione peroxidase enzyme,GSH-px)和超氧化物歧化酶(superoxide dismutase enzyme,SOD)活力,骨髓细胞周期变化。结果单纯亚低温组与正常对照组统计学指标差异均无统计学意义(P>0.05)。亚低温组骨髓有核细胞数在辐照后3 d和7 d[(25±1)×107个、(180±55)×107个]均低于氨磷汀组[(79±6)×107个、(270±19)×107个],差异有统计学意义(P<0.05)。骨髓病理组织变化亚低温和氨磷汀变化相似。辐照后6 h,亚低温组SOD活力[(48.39±3.82)U/m L]高于单纯照射组[(33.86±3.28)U/m L]和氨磷汀组[(32.92±2.39)U/m L],差异有统计学意义(P<0.01),亚低温组GSH-px活力[(732.98±121.69)U/m L]高于氨磷汀组[(475.67±76.04)U/m L],差异有统计学意义(P<0.05);G2/M期细胞氨磷汀组[(17.58±1.66)U/m L]相对亚低温组[(8.52±2.18)U/m L]更高(P<0.05)。辐照后24 h,亚低温组和氨磷汀组丙二醛含量、SOD活力和GSH-px活力差异无统计学意义(P>0.05)。结论亚低温不影响小鼠的健康状况,亚低温在机体早期抗氧化及调整细胞周期方面有更好的防护效果,而氨磷汀能更快地提高骨髓有核细胞数。Objective Amifostine,as a pan-cell radioprotective agent,has gained a clinical application in radiotherapy and chemotherapy,but may cause quite a few adverse reactions. This study aims to compare the effects of mild hypothermia and amifostine on radiation-induced injury in mice. Methods A total of 175 BALB/C male mice were randomly divided into 5 groups of equal number: normal control,mild hypothermia alone,irradiation alone,amifostine,and mild hypothermia. The animals in the irradiation alone,mild hypothermia,and amifostine groups were exposed to whole body irradiation of60Coγ ray at 6 Gy,those in the mild hypothermia group treated with mild hypothermia for 6 hours immediately after irradiation,those in the mild hypothermia alone group intervened with mild hypothermia for 6 hours after sham irradiation,and those in the amifostine group injected intraperitoneally with amifostine at 0.5 hour before irradiation. Then,we observed histopathological changes in the bone marrow,counted the nucleated cells in the bone marrow at 1,3,7,14,21,and 28 days after irradiation,measured the content of malondiadehyde( MDA) and the activity of superoxide dismutase enzyme( SOD) and glutathione peroxidase enzyme( GSH-px) in the serum,and detected the cell cycle in the bone marrow at 6 and 24 hours after irradiation. Results There were no statistically significant differences between the normal control and mildhypothermia alone groups( P>0.05). The numbers of nucleated cells were markedly lower in the mild hypothermia than in the amifostine group at 3 days( [25±1] vs [79±6]×107,P<0.01) and 7 days( [180±55]vs [270±6]×197,P<0.05) after irradiation. Histopathological changes of the bone marrow in the mild hypothermia group were similar to those in the amifostine group. At 6 hours after irradiation,the activity of SOD was remarkably higher in the mild hypothermia than in the irradiation alone and amifostine groups( [48.39±3.82]vs [33.86±3.28]and [32.92±2.39]U/mL,P<0.01),and so was that of GSH-px in the mild hypothermia than in t

关 键 词：亚低温 氨磷汀 辐射防护 

分 类 号：R122.2[医药卫生—环境卫生学]