铜绿微囊藻和小球藻对水环境pH的影响  

Effects of Microcystis aeruginosa and Chlorella pyrenoidosa on water environment pH

作　　者：苏发文 高鹏程 来琦芳[1] 么宗利[1] 陆建学[1] 周凯[1] 仲启铖[1] 郑亮[1] 王慧[1] 

机构地区：[1]中国水产科学研究院东海水产研究所,咸淡水养殖工程技术中心,上海200090 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《中国水产科学》2016年第6期1380-1388,共9页Journal of Fishery Sciences of China

基　　金：中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院东海水产研究所,2014T04);上海科技兴农[沪农科攻字(2015)第1-6号]

摘　　要：以盐碱池塘优势微藻铜绿微囊藻(Microcystis aeruginosa)、小球藻(Chlorella pyrenoidosa)为研究对象,采用正交实验方法,研究不同温度(20℃,25℃,30℃)和光照强度(2000 lx,4000 lx,6000 lx)组合条件下两种微藻对水环境pH的影响。结果显示,处于对数生长期的铜绿微囊藻和小球藻均能使水环境pH上升。在本实验范围内,不同温度和光照强度组合条件下,铜绿微囊藻的生长均能使水环境pH显著上升至9.50以上,在温度25℃、光照强度2000 lx条件下,藻密度达最大值1.1×10~7 cells/m L,水环境pH也达到峰值10.83;小球藻生长亦能使水环境pH的上升,并随温度升高、光照强度增强而增大,在温度30℃、光照强度6000 lx条件下,藻密度达最大值8.1×10~6 cells/m L,水环境pH也达到峰值7.73。通过ANCOVA分析,水环境pH和藻细胞密度呈正相线性相关,铜绿微囊藻水环境pH和藻细胞密度相关系数R^2=0.904,小球藻水环境pH和藻细胞密度相关系数R^2=0.903。与小球藻相比,同等藻密度的铜绿微囊藻更易使水环境pH显著上升(P<0.01),是池塘养殖水体pH偏高的主要原因之一,本研究旨在通过控制藻相方式进行水环境调控,从而防止池塘pH偏高提供了基础数据。With the continuous development and utilization of saline-alkali land, increasing quantities of saline-alkali water are being used for fisheries. However, the high pH value of the saline-alkali water environment is always a major factor restricting such aquaculture. Previous studies have revealed that the water environment pH value is mainly a result of the impact of CO2 balance system （CO3^2--HCO3^-CO2）; the CO2 balance system and activities of algae are also closely related. Microcystis aeruginosa and Chlorella pyrenoidosa, two dominant mi- croalgae species in saline-alkali aquaculture ponds, are of great importance in feed, nutrition and the aquaculture industry. Most studies have focused on the growth and reproduction of M. aeruginosa and C. pyrenoidosa, and only a few have reported on their relationship to the pH value of the water environment. In this study, anorthogonal experiment was designed to study the effects of temperature （20℃, 25℃ and 30℃） and light intensity （2000 lx, 4000 lx and 6000 lx） on the regulation of environmental pH by the growth of M. aeruginosa and C. pyrenoidosa. The results showed that temperature, light intensity and their： interactions.had a significant effects on the pH value of the algal medium, and there was a clear increase in pH during the exponential growth phase of these species. The water environment pH value of the M. aeruginosa medium generally increased by more than 9.50 as that alga grew under various temperature and light intensity conditions. The highest pH value of this medium was observed at 2000 1x and 25℃. Under these conditions, the water environment pH value of the M. aeruginosa medium reached its maximum value of 10.83 on the 9th day and the maximum algal density of 1.1 × 10^7 cells/mL on the 10th day. The highest pH value of the C pyrenoidosa medium was observed at 6000 Ix and 30℃. With the temperature and light intensity increasing, the maximum pH value and algal density were 7.73 and 8.0×10^6cells/mL, respectively, on thel0th day. A

关 键 词：铜绿微囊藻 小球藻 温度 光照强度 PH 

分 类 号：S912[农业科学—水产科学]