DOP对鲤鱼的遗传毒性及肝脏抗氧化系统的影响  

Effects of DOP on the Genetic Toxicity and Liver Antioxidant System in Carps

作　　者：李凤芝 

机构地区：[1]山东省单县杨楼镇孟寨中学,山东单县274300

出　　处：《安徽农业科学》2016年第28期130-132,共3页Journal of Anhui Agricultural Sciences

基　　金：山东省自然科学基金项目(ZR2010CM046)

摘　　要：[目的]研究邻苯二甲酸二辛酯(DOP)对鲤鱼的遗传毒性及抗氧化性能的影响,为评价DOP的生物学毒性及水环境生态风险提供理论依据。[方法]应用吉姆萨染色和试剂盒方法,研究0.3、1.5、7.5 mg/L的DOP 24 h暴露对鲤鱼红细胞核异常率和肝脏超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性及丙二醛(MDA)含量的影响。[结果]在DOP试验浓度范围内,红细胞微核率、核异常率和总核异常率均随着DOP浓度的升高而升高,且微核率在7.5 mg/L DOP组极显著高于对照组。SOD活性则表现为低促高抑。0.3 mg/L DOP组POD活性显著高于对照组,而1.5 mg/L DOP、7.5 mg/L DOP组POD活性无明显变化。MDA含量随着DOP浓度的升高而升高,且各浓度DOP组MDA含量均显著高于对照组。[结论]一定浓度的DOP对鱼类具有遗传毒性,并能损伤抗氧化防御系统。Objective] To study the effects of DOP on the genetic toxicity and oxidation resistance of carps,and to provide theoretical basis for evaluating the biological toxicity and ecological risk to water environments of DOP.[Method] The effects of exposing with DOP （0.3,1.5,7.5 mg/L） for 24 h on erythrocyte nuclei abnormal rate and activities of liver SOD and POD and the content of MDA by Giemsa staining and the kit method.[ Result] In the experimental concentration range of DOP,rate of micronucleus,nuclear anomalies and total nuclear anomaly on the eryth-rocyte were increased with the increase of DOP concentration,and the rate of micronucleus in the 7.5 mg/L DOP group was significantly higher than that in the control group.SOD activity was promoted in Low concentration and inhibited in high concentration.POD activity at 0.3 mg/L group was significantly higher,but it had no significant change at 1.5 and 7.5 mg/L DOP group compared with the control group.The content of MDA was increased with the increase of DOP concentration,and that in each DOP group was significantly higher than the control group.[ Conclu-sion] A certain concentration of DOP has a genetic toxicity and can damage the antioxidant defense system of fish.

关 键 词：邻苯二甲酸二辛酯 鲤鱼 微核 核异常 超氧化物歧化酶 过氧化物酶 丙二醛 Dioctyl Phthalate(DOP) CYPRINUS carpio Linnaeus(Carp) 

分 类 号：S965.116[农业科学—水产养殖]