HPLC法测定首乌益智胶囊中大黄素和大黄素甲醚的含量  

HPLC based content determination of emodin and emodin monomenthyl ether from Shouwuyizhi capsule

作　　者：丁晓彦[1] 刘青[1] 贾元印[1] 赵渤年[1] 

机构地区：[1]山东省中医药研究院,山东省中药质量标准研究重点实验室,山东济南250014

出　　处：《山东科学》2016年第5期36-40,共5页Shandong Science

基　　金：山东省自主创新及成果转化专项(20140ZZCX2303)

摘　　要：建立了高效液相色谱法（HPLC）同时测定首乌益智胶囊中大黄素和大黄素甲醚含量的方法。采用C18（250mm×4．6mm，5μm）色谱柱，以甲醇．0．1％磷酸水（82：18，WV）为流动相，检测波长为289nm，流速为1．0mL／min，柱温30℃。测得大黄素在0．019～0．19μg（R=0．9999，n=5）、大黄素甲醚在0．021—0．21μg（R=1．000O，n=5）线性关系良好；大黄素和大黄素甲醚的平均回收率分别为98．58％、100．01％，相对标准偏差分别为2．66％、1．15％。研究表明，该方法专属性强、结果准确，可作为同时测定首乌益智胶囊中大黄素和大黄素甲醚含量的方法。We constructed a HPLC based content determination method for emodin and emodin monomenthyl ether from Shouwuyizhi capsule. Samples were separated by C18（250 nm×4.6 nm, 5 μm） column with methanol-0.1% phosphoric acid water （ 82： 18, V/V） as mobile phase. Determination 1.0 mL/min, and column temperature of 30 ℃. Better conditions were determination wavelength of 289 rim, flow rate of near relationship exists in the range 0.019-0.19 μg（R=0.999 9,n=5） for emodin, and in the range 0.021-0.21 μg（ R= 1.000 0, n= 5）for emodin monomenthyl ether. Their average recovery rates are respectively 98.58% and 100.01%, and RSDs are 2.66% and 1.15%. Results show that the method has strong specificity and accuracy, so it can be applied to simultaneous content determination of emodin and emodin monomenthyl ether from Shouwuyizhi capsule.

关 键 词：首乌益智胶囊 大黄素 大黄素甲醚 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]