甲泼尼龙作用下特异性免疫效应细胞抑制乙肝病毒复制的体外研究  

Effects of specific immune effector cells in hepatitis B virus replication by methylprednisolone in vitro

作　　者：王绕绕 杨柳[1] 赵华[1] 尹明丽[1] 郑卫萍[1] 沈中阳[1] 宋红丽[1] 

机构地区：[1]天津医科大学一中心临床学院,300070

出　　处：《实用医学杂志》2016年第21期3489-3493,共5页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:81270528;81170444;81441022);天津市卫生局科技基金资助项目(编号:2011KY11;2014KZ023)

摘　　要：目的:探讨体外甲泼尼龙(MP)作用下,特异性免疫效应细胞(IECs)对乙肝病毒稳定复制的HepG2.2.15乙型肝炎病毒(HBV)复制的影响及机制。方法:分离人树突状细胞、负载HBs Ag诱导成熟;后与自体淋巴细胞共孵,形成IECs与HepG2.2.15共培养,MP处理各组细胞,采用生物化学、PCR、流式细胞仪和ELISA等方法检测肝功能、HBV DNA、T细胞亚群和炎症相关因子。结果:48 h后,IECs作用于HepG2.2.15,可抑制上清HBV DNA表达(P<0.01),IFN-γ升高,IL-10、IL-17、IL-23下降(P<0.01)。MP作用于HepG2.2.15,可抑制HBV DNA表达(P<0.01);MP使IECs对HBV DNA抑制作用减弱,但仍较单纯HepG2.2.15的HBV DNA降低。结论 :IECs可显著抑制HepG2.2.15的HBV DNA表达;MP作用下,IECs仍能抑制HepG2.2.15的HBV DNA表达,发挥抗病毒作用;其酶学和合成功能无明显变化。Objective To investigate the effects ofspecific immune effector cells （IECs） on HepG2.2.15 by methylprednisolone （MP）. Methods Dendritic cells were isolated andcuhured with lymphocytes to form IECs, then they were co-cultured with HepG2.2.15. We chose MP to treat with cells. The levels of HBV DNA were measured by PCR. Inflammatory cytokines levels were detected by ELISA and T cell subsets were detected by flow cytometry analysis. Results After 48h, IECs refrained HepG2.2.15 in HBV DNA （P 〈 0.01）. The levels of IFN-γ was increased, while IL-10,IL-17 and IL-23 were decreased （P 〈 0.01 ）. After using MP to treat with cells, HBV DNA levels （P 〈 0.01）. However, MP could suppress IECs to attenuate the levels of HBV DNA, but the effect with MP was still better than HepG2.2.15 alone. Conclusions IECs can suppress the levels of HBV DNA by MP. In addition, there is no adverse reactions to liver function by MP.

关 键 词：乙型肝炎病毒 树突状细胞 HEPG2.2.15 特异性免疫效应细胞 甲泼尼龙 

分 类 号：R512.62[医药卫生—内科学]