呋喃二烯长循环脂质体的制备与表征  

Preparation and characterization of furanodiene long-circulation liposomes

作　　者：杨秀春[1] 张建梅[1] 李琳 王东凯[1] 

机构地区：[1]沈阳药科大学,沈阳110016 [2]宁夏启元国药有限公司,银川750100

出　　处：《中国新药杂志》2016年第21期2500-2505,共6页Chinese Journal of New Drugs

摘　　要：目的:制备呋喃二烯长循环脂质体并进行表征。方法:采用薄膜超声分散法制备呋喃二烯长循环脂质体,以包封率和粒径为评价指标,通过单因素-正交设计优化脂质体处方及工艺。结果:优化后的处方和制备工艺为:水化温度为55℃,磷脂浓度为3.5%,磷脂与胆固醇比例为12∶1,脂药比为15∶1,DSPEMPEG2000摩尔百分比为5%。此条件下制备的脂质体包封率为90.6%,平均粒径为113.4 nm,Zeta电位为-45.9 m V。结论:本实验所制备的呋喃二烯长循环脂质体包封率较高,粒度分布范围较窄,平均粒径较小,具有很好的应用前景。Objective： To prepare and characterize long-circulation liposomes containing furanodiene.Methods： Liposomes were prepared by a thin-film ultrasonic dispersion method. With encapsulation efficiency and particle size as indexes,the formulation and preparation process was optimized by the single factor experiment and orthogonal test. Results： The optimized formulation was as follows： the hydration temperature was 55 ℃,the concentration of phospholipid was 3. 5%,weight ratio of phospholipid to cholesterol was 12 ∶ 1,weight ratio of phospholipid to drug was 15 ∶ 1,and molar ratio of DSPE-MPEG2000 was 5%. The encapsulation efficiency of furanodiene liposomes was 90. 6% with an average diameter of 113. 4 nm and the Zeta potential of-45. 9 m V.Conclusion： The prepared furanodiene liposomes have higher entrapment efficiency, and lower particle size distribution. And smaller particle size,showing a great potential for further investigation.

关 键 词：呋喃二烯 长循环脂质体 薄膜超声分散法 正交试验 

分 类 号：R943.4[医药卫生—药剂学]