Sysmex XN-1000血细胞分析仪计数低值白细胞性能的评估  

Performance evaluation of XN-1000 Sysmex blood cell count of low value of white blood cell

作　　者：蒋清清[1] 马萍[1] 丁爽[1] 

机构地区：[1]徐州医学院附属医院检验科,江苏徐州221003

出　　处：《国际检验医学杂志》2016年第21期3002-3003,3006,共3页International Journal of Laboratory Medicine

摘　　要：目的探讨Sysmex XN-1000（后简称XN-1000）全自动血细胞分析仪检测白细胞计数（WBC）及分类的准确性。方法收集该院2015年1~4月门诊及住院患者及健康体检者共206例,根据人工镜检检测WBC结果分为高值组（WBC〉10.0×109/L）,健康组（4.0×109/L0.05）,结果具有正相关性。低值组中,XN-1000检测结果准确性与XE-5000差异无统计学意义（P〉0.05）,但是白细胞分类结果显著高于XE-5000（P〈0.05）;对于异常细胞,XN-1000和XE-5000不能进行有效分类,仍需人工镜检进行分类。结论 XN-1000全自动血细胞分析仪在WBC与XE-5000及人工镜检比较无差异统计学意义,在测定白细胞分类上XN-1000较XE-5000更准确（XN-1000合格率91%,XE-5000合格率63%）,但仍不能完全替代人工镜检。对于异常细胞,XN-1000不能有效分类,需与人工镜检结合。Objective To investigate the accuracy of XN-1000 automatic blood cell analyzer in detecting of white blood cell（WBC）count and classification.Methods A total of 206 cases including outpatients,inpatients and health people were collected in the hospital from January to April 2015.According to manual microscopy detection of white blood cell count results,the cases were divided into three groups：high value of white blood cell（WBC was more than 10.0×109/L）group,normal value of white blood cells group（WBC was more than 4.0×109/L but less than 10.0×109/L）and low value of white blood cell（WBC was more than1.5×109/L but less than 4.0×109/L）group.WBC count and classification were detected with Sysmex XN-1000 and Sysmex XE-5000 respectively.The results and the correlation analysis of the three methods was compared by the statistical software.ResultsThe difference of WBC count between between high value group and normal value group was not statistically significant（P〉0.05）,and the results detected by Sysmex XN-1000 was positively correlated with that of Sysmex XE-5000 and manual microscopy detection.In low value group,the accuracy of the test results between Sysmex XN-1000 and Sysmex XE-5000 had no significant difference（P〉0.05）.However,the accuracy of the WBC classification by Sysmex XN-1000（91%）was significantly higher than Sysmex XE-5000（63%,P〈0.05）.Sysmex XN-1000 and XE-5000couldn′t classify abnormal cells effectively.The classification abnormal cells still needed to artificial microscopy classification.Conclusion The classification of WBC by Sysmex XN-1000 is more accurate than XE-5000,but it still can not completely replace the manual microscopy,especially for abnormal cells.The classification of abnormal cells should combine the manual microscopy.

关 键 词：XN-1000 低值白细胞 人工镜检 

分 类 号：R446.11[医药卫生—诊断学]