大果水晶梨褐色果皮突变体木质素合成相关基因的组织特异性表达分析  

Tissue Specificity Express Analysis of Enzyme Genes Related to Lignin Biosynthesis in Suisho Pear Brown Pericarp Mutant

作　　者：刘炳旭[1] 于凤鸣[1] 张立彬[2] 武军凯 宋立琴[2] 肖啸[2] 杜晓东[3] 

机构地区：[1]河北科技师范学院生命科技学院,河北秦皇岛066004 [2]河北科技师范学院园艺科技学院,河北秦皇岛066004 [3]河北省农林科学院农业信息与经济研究所,河北石家庄050051

出　　处：《华北农学报》2016年第5期28-34,共7页Acta Agriculturae Boreali-Sinica

基　　金：河北省教育厅重点项目(No.ZH2012005)

摘　　要：为研究木质素合成相关基因(PAL1、PAL2、CAD、POD、4CL)在大果水晶梨褐色果皮突变体中的表达特性,并为研究梨褐色果皮形成机制奠定基础。按照RNA试剂盒法提取果皮、花、叶片、果肉、枝条韧皮部中总RNA;利用DNAMAN和Primer 5.0软件设计特异引物;使用实时荧光定量PCR技术研究基因的表达。结果表明,PAL1、PAL2、CAD、POD、4CL的最高相对表达量均出现在果皮中;果皮和果肉中POD的相对表达量极显著高于其他基因,花、叶片和枝条韧皮部中CAD的相对表达量极显著高于其他基因。综上所述,大果水晶梨褐色果皮突变体木质素合成相关的5个酶基因在果皮、果肉、叶片、花和枝条韧皮部中的表达具有明显的组织特异性,这些基因可能与大果水晶梨褐色果皮的形成关系密切。In order to reveal the expression characteristic of genes（ PAL1,PAL2,CAD,4CL,POD） related to lignin biosynthesis in brown pericarp mutant of Suisho pear,and provide the basis for further studying pear brown pericarp formation mechanism. RNA was extracted from different tissues according to RNA extraction kit（ EASY spin,Biomed）,primers were designed with Primer 5. 0 and DNAMAN,expression pattern of genes were analyzed by QRT-PCR. Among the different tissues and organs,the highest relative expression of PAL1,PAL2,CAD,4CL,POD gene appeared in peels. In peels and pulps,the relative expression of POD gene was extremely significantly higher than the other genes. In flowers,leaves and branches phloem,the relative expression of CAD was extremely significantly higher than the other genes. The study suggested that the expression pattern of PAL,CAD,4CL and POD had significant tissue-specific in the five selected tissues and organs,and these genes may be associated with the formation of Suisho pear brown peel closely.

关 键 词：大果水晶梨褐色果皮突变体 木质素合成关键酶 基因 反转录-聚合酶链式反应(RT-PCR) 组织特异性表达 

分 类 号：Q78[生物学—分子生物学] S661.03[农业科学—果树学]