新型罗丹明B荧光探针的合成及其对Hg^(2+)的识别研究  

A Novel Rhodamine B Fluorescent Probe for Hg^(2+): Synthesis and Evaluation

作　　者：肖慧丰 张敏[1] 刘杰[1] 韩志湘[2] 仰榴青[1] 吴向阳[2] 

机构地区：[1]江苏大学化学化工学院,镇江212013 [2]江苏大学环境与安全工程学院,镇江212013

出　　处：《有机化学》2016年第10期2413-2418,共6页Chinese Journal of Organic Chemistry

基　　金：江苏省自然科学基金(No.BK20140536)资助项目~~

摘　　要：以罗丹明B酰肼1和苯基乙二醛缩合反应得到席夫碱2,再经硼氢化钠还原得到新型罗丹明B衍生物R,采用~1H NMR、^(13)C NMR、ESI-MS、元素分析以及X-ray单晶射线对其结构进行了验证.研究结果表明,化合物R作为荧光探针能选择性地识别Hg^(2+),且受常见金属离子的干扰较小.探针R的荧光强度与Hg^(2+)浓度(5×10^(-7)~2.5×10^(-6)mol/L)呈良好的线性关系,并在MGC-803活细胞中实现了Hg^(2+)的成像.A novel fluorescent probe R based on rhodamine B was synthesized by the condensation of rhodamine B hydrazide and phenylglyoxal monohydrate, followed by reduction with NaBH4, and its structure was characterized by ^1H NMR, ^13C NMR, ESI-MS, elemental analysis, and X-ray single analysis. It exhibited very strong fluorescence responses to Hg^2＋ with remarkably high selectivity for Hg^2＋ over other metal ions. When the concentration of Hg^2＋ was in the range of 5×10^-7-2×10^-6 mol/L, there was a good linearity between the fluorescence intensity and the concentration of Hg^2＋. The fluorescence imaging experiments of Hg^2＋ in MGC-803 living cells revealed its potential application in biological system.

关 键 词：荧光探针 罗丹明B 汞离子 细胞成像 

分 类 号：X830[环境科学与工程—环境工程] O657.3[理学—分析化学]