肠道病毒71型感染SK-N-SH神经细胞的microRNA表达谱差异分析  

MicroRNA expression profile of SK-N-SH neural cells infected with human enterovirus 71

作　　者：茅凌翔[1,2] 吴静[3] 沈俐[4] 杨静[4] 陈建国[2] 许化溪[1] MAO Ling-xiang WU Jing SHEN Li YANG Jing CHEN Jian-guo XU Hua-xi(School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China)

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212013 [2]江苏大学附属人民医院检验科,江苏镇江212002 [3]江苏大学医学院,江苏镇江212013 [4]镇江市疾病预防控制中心检验科,江苏镇江212001

出　　处：《中国病毒病杂志》2016年第2期120-124,共5页Chinese Journal of Viral Diseases

基　　金：镇江市卫生科技重点专项(SHW2015014)

摘　　要：目的分析人肠道病毒71型(EV-A71)感染神经母细胞瘤来源的SK-N-SH细胞的microRNA(miRNA)表达谱差异。方法用EV-A71病毒感染人SK-N-SH细胞,6h后收获总RNA,基因芯片检测感染和未感染EV-A71的神经细胞miRNA表达谱,并用实时荧光定量PCR方法 (qRT-PCR)进行验证。结果比较EV-A71感染与未感染的SK-N-SH细胞miRNA的表达谱,SK-N-SH细胞被EV-A71感染后有26种miRNA表达显著上调,40种miRNA表达显著下调。结论 EV-A71感染显著改变了SK-N-SH神经细胞的miRNA表达谱,可作为EV-A71感染机制深入研究的实验基础。Objective To analyze the expression profile of miRNA in neural cell line SK-N-SH, infected with human enterovirus 71 （EV-A71）. Methods The total RNA of SK-N-SH neural cells infected with EVA71 for 6 hours was harvested, miRNA chip was used to detect the miRNA expression profile of cells, which was then verified by quantitative real-time PCR （qRT-PCR）. Results A total of 26 miRNAs were up-regulated while 40 were down-regulated apparently in SK-N-SH cells infected with EV-A71 compared with non-infected cells. Conclusions The infection of EV-A71 can significantly change the miRNA expression profile of SK-N- SH cells, which may be used as the experimental basis for the further study of the mechanism of EV-A71 infection.

关 键 词：肠道病毒71型 神经细胞 MIRNA芯片 

分 类 号：R373[医药卫生—病原生物学]