犬卵透明带3(CZP3)DNA疫苗免疫有效降低小鼠生育能力  

Canine zona pellucida 3(CZP3) DNA vaccination reduces mouse fertility effectively

作　　者：王颖[1] 张贝贝[1] 张冀[1] 罗娇娇[1] 阿地拉.艾皮热 张富春[1] WANG Ying ZHANG Beibei ZHANG Ji LUO Jiaojiao ADILA Aipire ZHANG Fuchun(Xinjiang Key Laboratory of Biological Resource and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, Chin)

机构地区：[1]新疆大学生命科学与技术学院新疆生物资源基因工程重点实验室,新疆乌鲁木齐830046

出　　处：《细胞与分子免疫学杂志》2016年第10期1331-1335,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：新疆维吾尔自治区科技支疆项目(201591134)

摘　　要：目的构建以犬卵透明带3(CZP3)为靶抗原的DNA避孕疫苗,并对其免疫效果及抗生育能力进行初步评价。方法提取犬卵巢总RNA,反转录PCR扩增CZP3基因,利用Prot Scale、TMHMM、Signal P、Inter Pro Scan、PREDICT PROTEIN、同源模建等生物信息学方法对CZP3基因进行分析,构建携带目标抗原CZP3的DNA疫苗pc DNA-CZP3,进行小鼠免疫实验,对DNA疫苗的免疫效果、抗生育水平进行综合评价。结果 CZP3基因序列共编码426个氨基酸,其在N端和C端各有1个疏水区,N端前22个氨基酸为信号肽,C端有1个由细胞外到细胞内的跨膜螺旋。CZP3有8个B细胞表位。DNA避孕疫苗pc DNA3-CZP3能够诱导小鼠产生较高的抗体水平,免疫组小鼠平均产仔数显著低于空白对照组和阴性对照组。结论成功构建能显著降低小鼠生育能力的犬避孕DNA疫苗pc DNA3-CZP3。Objective To construct a DNA contraception vaccine targeting canine zona pellucida 3（ CZP3） antigen and assess the immunological efficacy and contraceptive effect of the vaccine. Methods The CZP3 gene was amplified from total RNA of canine ovary by reverse transcription PCR and analyzed by bioinformatics,such as Prot Scale,TMHMM,Signal P,Inter Pro Scan,PREDICT PROTEIN,homology modeling,etc. The constructed DNA vaccine pc DNA-CZP3 was used to vaccinate mice,and then its immune effect and contraceptive effect were evaluated in the mice. Results The CZP3 gene had 426 amino acids with two hydrophobic regions at its N-terminal and C-terminal,respectively. The top 22 amino acids at the N-terminal of the CZP3 was the signal peptide and there was a transmembranous helix from extracellular to intracellular at the C-terminal. CZP3 also had 8 B cell epitopes. The DNA contraception vaccine pc DNA3-CZP3 induced high levels of antibody and lower average litter size of mouse compared with the blank and negative control groups significantly. Conclusion The canine contraception DNA vaccine pc DNA-CZP3 has been successfully constructed and it can reduce the mouse fertility remarkably.

关 键 词：犬卵透明带3(CZP3) 序列分析 DNA疫苗 免疫避孕 

分 类 号：R169.41[医药卫生—公共卫生与预防医学] R392.11