Isolation and Stucture Elucidation of Diterpenes from Wedelia prostrata and Their Cytotoxicity Activities  

作　　者：CHEN Qing LIN Jianhong ZHAO Dan ZHU Xuan 

机构地区：[1]Department of Pharmacy, School of Pharmaceutical Science, Xiamen University, Xiamen 361102, P. R. China

出　　处：《Chemical Research in Chinese Universities》2016年第5期792-796,共5页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China（No.31300285）, the Natural Science Foundation of Fujian Province, China（No.2015J01348）, the Specialized Research Fund for the Doctoral Program of Higher Education of China （No.20110073120072） and the Fujian Province Development Guidance（Key） Project, China（No.2014Y0074）.

摘　　要：In this paper, a new diterpene together with seven known diterpenes was isolated from Wedelia prostrata. The chemical structure of the new compound was elucidated via 1D and 2D nuclear magnetic resonance（NMR） tech- niques and mass spectrometry and identified to be 3a-phenylpropionoyloxy-ent-kaur-16-en-oic acid（l）. The isolated diterpenes were tested for their cytotoxicity activities via 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bro- mide（MTT） assay. The results show that compounds 1, 2, 3, 4 and 6 exhibit different levels of cytotoxic activities. Especially, compound 2 shows significant cytotoxicity toward HeLa and A549 cell lines（IC50=6.14 and 8.76 μmol/L）.In this paper, a new diterpene together with seven known diterpenes was isolated from Wedelia prostrata. The chemical structure of the new compound was elucidated via 1D and 2D nuclear magnetic resonance（NMR） tech- niques and mass spectrometry and identified to be 3a-phenylpropionoyloxy-ent-kaur-16-en-oic acid（l）. The isolated diterpenes were tested for their cytotoxicity activities via 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bro- mide（MTT） assay. The results show that compounds 1, 2, 3, 4 and 6 exhibit different levels of cytotoxic activities. Especially, compound 2 shows significant cytotoxicity toward HeLa and A549 cell lines（IC50=6.14 and 8.76 μmol/L）.

关 键 词：Wedelia prostrata 3a-Phenylpropionoyloxy-ent-kaur-16-en-oic acid Chemical constituent Cytotoxicityactivity 

分 类 号：O[理学]