中空硅纳米药物递送体系的线粒体靶向性探究  

Hollow Silica Nanoparticles for Mitochondria-targeted Drug Delivery

作　　者：王艳妨 包暄文 宋小杰[1] 王冰[1] 张英[1] 孙广炜[1] 马小军[1] 

机构地区：[1]中国科学院大连化学物理研究所,辽宁大连116023 [2]大连医科大学第一附属医院,辽宁大连116000

出　　处：《现代生物医学进展》2016年第27期5201-5205,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(31470944;21576254;31271055)

摘　　要：目的:探究中空硅纳米粒在细胞线粒体内的定位。方法:以阿霉素为模型药物及荧光影像分子,通过激光共聚焦显微镜,考察中空硅纳米粒与线粒体分子探针在线粒体内的共定位。通过差速离心法提取线粒体,进一步确认硅纳米粒在线粒体内的聚集。结果:与线粒体分子探针为共定位参照,中孔硅纳米粒在线粒体中分布的皮尔逊系数为0.758±0.033,线粒体提取结果显示,进入细胞的纳米粒有90.01±3.89%分布于线粒体。结论:中空硅纳米粒具有自发聚集于线粒体的能力,可以作为药物靶向递送载体,为线粒体类疾病的治疗提供新的平台。Objective： To investigate the location of hollow silica nanoparticles in cells. Methods： Taken doxorubicin hydrochloride as the modal drug and fluorescence imaging molecular, the location of silica nanoparticles in cells were co-located with Mito-tracker Deep Red by laser scanning confocal microscope. The location of silica nanoparticles were further confirmed by isolation of mitochondrial and cytosolic extracts through differential centrifugation. Results： Compared with Mitotracker Deep Red, the Pearson’s Coefficient of the silica nanoparticles was 0.758±0.033, while the isolation of mitochondria showed 90.01±3.89% of the model drug distributed in mitochondria. Conclusions： The hollow silica nanoparticles can spontaneously gathered in mitochondria, which provides a new platform to mitochondria-targeted drug delivery system for mitochondria dysfunctional diseases.

关 键 词：中空硅纳米粒 线粒体靶向 药物递送 

分 类 号：R318.08[医药卫生—生物医学工程] R94[医药卫生—基础医学]