广东部分地区猪流行性腹泻病毒流行病学调查  

Molecular characterization and epidemiological survey of porcine epidemic diarrhea virus in some areas of Guangdong

作　　者：白小磊[1,2] 崔进[1,2] 崔甜甜[1,2] 陈耀[1,2] 石庆伟[1,2] 冯嘉萍 王林川[2] 张桂红[1,2] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]国家生猪种业工程技术研究中心,广东广州510642

出　　处：《中国兽医学报》2016年第11期1823-1828,1863,共7页Chinese Journal of Veterinary Science

基　　金：国家生猪产业技术体系基金资助项目(CARS-36)

摘　　要：本试验对临床上猪腹泻相关的病原进行了检测并对广东部分地区的猪流行性腹泻病毒(porcine epidemic diarrhea virus,PEDV)展开了分子流行病学的调查。对采集自广东地区的14个规模化猪场的19份腹泻病料进行了RT-PCR检测,并对检出的12株PEDV的COE、ORF3、M以及N基因进行测序和遗传进化分析。结果显示,临床的腹泻案列多为混合感染所致,检出的主要病原有猪传染性胃肠炎病毒(TGEV)、PEDV、猪嵴病毒(PKV)、猪A群轮状病毒(PoRV)以及牛流行性腹泻病毒(BVDV)。与参考毒株CV777相比12株PEDV的COE、ORF3、M、N基因均存在不同程度、不同位点的氨基酸突变。COE、ORF3、M以及N基因的遗传进化分析显示:广东地区的12株PEDV毒株属于同一个分支彼此间的亲缘关系较近但与CV777疫苗毒株以及2012年之前广东地区分离的毒株CHGD-01、CH-GDGZ-2012、GD-1以及GD-A等毒株分属于2个不同的分支,亲缘关系相对较远。结果表明,广东地区现阶段流行的PEDV优势毒株与2012年之前相比可能已经发生了改变,CV777疫苗毒株的免疫效果如何还有待于通过更多的临床实验来验证。To investigate the epidemiological and prevalence of porcine diarrheal disease, 19 of clinical samples were collected from 14 pig farms suspected porcine diarrheal disease and were detected by RT-PCR. The results show that the diarrhea in most pig farms was mixed infection, the main virus were porcine epidemic diarrhea viruses, porcine rotavirus, bovine viral diarrhea virus, trans- missible gastroenteritis virus, and Porcine kobuvirus. Sequence comparison and analysis results showed that COE,ORF3, M and N genes of 12 strains displayed different amino acid site mutation in different degree compared with CV777 strain. Neighbor - joining trees constructed by using COE,ORF3,M and N genes showed that the 12 isolates had a close relationship with each other, and were distant from the vaccine strain CV777 and CHGD-01, CH-GDGZ-2012, GD-1 and GD-A isolates collected in Guangdong before 2012. All these results showed that the PEDV strains prevailing in Guangdong have changed compared to isolates before 2012, the immune efficiency of CV777 vaccine strain remains to be verified by more clinic experiments.

关 键 词：猪腹泻 PEDV 分子流行病学 亲缘关系 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]