复方氟苯尼考注射液体外抑菌试验及对人工感染巴氏杆菌仔猪的保护效果  

Antibacterial activity in vitro and protective experiment of compound florfenicol injection against artificially infected Pasteurella multocidain piglings

作　　者：班赟 叶文初[1] 袁富威 杨亚军[2] 李剑勇[2] 刘敏[1] 李引乾[1] 

机构地区：[1]西北农林科技大学动物医学学院,陕西杨凌712100 [2]中国农业科学院兰州畜牧与兽药研究所农业部兽用药物创制重点实验室/甘肃省新兽药工程重点实验室,甘肃兰州730050

出　　处：《中国兽医学报》2016年第11期1908-1915,共8页Chinese Journal of Veterinary Science

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(1610322012005);公益性行业(农业)科研专项(201303038-4);甘肃省科技重大专项计划项目(1302NKDA024)

摘　　要：为了研究复方氟苯尼考注射液的体外抗菌活性及对人工感染巴氏杆菌仔猪的保护效果。本研究采用体外抑菌试验采用试管二倍稀释法,测定复方氟苯尼考注射液和单方氟苯尼考注射液对多杀性巴氏杆菌、副猪嗜血杆菌、猪传染性胸膜肺炎放线杆菌及链球菌的最小抑菌浓度(MIC)。体内治疗试验是将复方氟苯尼考注射液分为20、40、60mg/kg 3个剂量组与氟苯尼考对照组(40mg/kg)以及氟尼辛葡甲胺对照组(3.65mg/kg)以颈部肌肉注射治疗人工感染多杀性巴氏杆菌的仔猪。体外抑菌试验结果显示,复方氟苯尼考注射液对多杀性巴氏杆菌、副猪嗜血杆菌、猪传染性胸膜肺炎放线杆菌及链球菌的MIC分别为0.59、1.09、0.59、2.12mg/L;氟苯尼考注射液对多杀性巴氏杆菌、副猪嗜血杆菌、猪传染性胸膜肺炎放线杆菌及链球菌的MIC分别为1.12、6.13、17.86、17.86mg/L。保护性试验结果显示,复方氟苯尼考注射液低、中、高剂量组增重率分别为(10.19±4.84)、(14.33±4.07)、(15.53±5.38)kg,死亡率分别为10%、10%、0,好转率分别为60%、90%、100%,治愈率分别为20%、90%、100%;氟苯尼考组的增重率为(9.90±4.25)kg;氟尼辛葡甲胺组的增重率为(8.02±3.83)kg。结果表明,复方氟苯尼考注射液对多杀性巴氏杆菌、副猪嗜血杆菌、猪传染性胸膜肺炎放线杆菌及链球菌的抑菌效果强于单方氟苯尼考注射液;复方氟苯尼考注射液对人工感染多杀性巴氏杆菌仔猪的保护作用也优于氟苯尼考注射液。建议本品的治疗剂量为颈部肌肉注射40mg/kg,每7d给药1次,连用2次,如症状严重可2d给药1次,连用2次。To study the antibacterial activity in vitro and explore the protective effect of compound florfenicol injection against artificially infected Pasteurella multocida in pigling. Minimal inhibitory concentration （MIC） of compound florfenicol injection against Pasteurella multocida, Haemophilus parasuis,Actinobacillus pleuropneumoniae and Streptococcus were detemined by two-fold dihution method in vitro. The piglings infected by Pasteurella multocida artificially were Carry out Aherapy in vivo. All the infected piglings were medicated via neck muscle injection with compound florfenicol injection groups （20,40,60 mg/kg）, florfenicol group （40 mg/kg）, flunixin meglumine group（3. 65 mg/kg）, respectively. The results of the antibacterial activity in vitro showed that MIC of Pasteurella multocida , Haemophilus parasuis , Actinobacillus pleuropneumoniae and Streptococcus for compound florfenicol injection was 0.59 mg/L, 1.09μg/mL,0.59 μg/mL,2.12 mg/L,respectively. The results of protective effect indicated that in compound flor-fenicol injection groups （20,40,60 mg/kg）, the increased rate of weight was （10. 19±4.84）, （14.33±4.07） ,（15.53±5.38） kg;the mortality were 10%,10%,0;the improvement rates was 60%,90%,100% and the cure rates was 20% ,90% ,100%. The increased rate of weight in florfenicol injection group was （9.90±4.25） kg and flunixin meglumine group was（8.02±3.83） kg. The antibacterial activity of Compound Florfenicol injection against Pasteurella multocida, Haemophilus parasuis , Actinobacillus pleuropneumoniae and Streptococcus were better than Florfenicol injection. The protective effect of compound florfenicol injection on artificial infection of Pasteurella rnultocida in piglings is better than that of florfenicol injection. It proposed that therapeutic dose of the compound florfenicol injection is 40 mg/kg via neck muuscle injection,one time every 7 days for two times ,or one time every 2 days for two times in severe case.

关 键 词：复方氟苯尼考注射液 MIC 仔猪 巴氏杆菌 

分 类 号：S859.7[农业科学—临床兽医学]