构树叶提取物体外抗病毒活性研究  

Study on Antiviral Activity of Broussonetia papyrifera Leaf Extracts in vitro

作　　者：杜柏槐 刘晓军[1] 陈绍红[2] 

机构地区：[1]广东海洋大学农学院,广东湛江524088 [2]广东海洋大学食品科技学院,广东湛江524088

出　　处：《安徽农业科学》2016年第29期144-146,共3页Journal of Anhui Agricultural Sciences

基　　金：广东省农业厅科技计划项目(0809035)

摘　　要：[目的]研究构树叶提取物的抗病毒活性。[方法]采用MTT法,观察构树叶的水、75%乙醇和50%丙酮提取物及不同给药方式对NDV、IBDV、ILTV和IBV等病毒感染的鸡胚成纤维细胞(CEF)活性的影响,探讨构树叶提取物体外抗病毒活性及其作用机制。[结果]乙醇和丙酮提取物能显著提高NDV感染的CEF细胞活性,丙酮提取物能显著提高ILTV和IBV感染的CEF细胞活性,但对IBDV诱导的CEF细胞病变无影响。经丙酮提取物预处理的CEF细胞对NDV或ILTV感染的抵抗力呈上升趋势。[结论]构树提取物中的有效成分可能通过阻断病毒对宿主细胞的识别和粘附发挥其抗病毒活性。[Objective] The present work was aimed to study the antiviral activity of Broussonetia papyrifera leaf extracts.[ Method] The MTT method was used to detect the effects of water, 75% ethanol and 50% acetone extracts of B.papyrifera leaf and different treatments on chick embryofibroblast（ CEF） infected by newcastle disease virus （ NDV ） , infectious laryngotracheitis virus （ ILTV ） , infectious bronchitis virus （IBV） and infectious bursal disease virus（IBDV） respectively, to explore the antiviral activity and mechanism of B.papyrifera leaf extracts. [ Result] The result showed that the vitality of the NDV infected CEF was significantly increased owing to ethanol and acetone extracts supple-mented in the cell medium, the vitality of CEF infected by ILTV or IBV were also significantly improved by acetone extract addition, whereas the cytopathic effect induced by IBDV was not alerted by B.papyrifera leaf extracts.Moreover, the vitality of CEF pretreated by acetone ex-tract, followed by NDV or ILTV infection, tend to be higher than that of other treatments.[ Conclusion] It can be inferred that the effective components in the B.papyrifera extract may exert the antiviral effect by blocking virus recognition and adhesion to the host cells.

关 键 词：构树叶 提取物 抗病毒活性 

分 类 号：S853.7[农业科学—临床兽医学]