P-cadherin在‘高山美利奴羊’胚胎皮肤毛囊基板形成过程中的表达规律  

Expression of P-cadherinin the process of formation in alpine merino sheep embryo skin follicle placode

作　　者：刘善博 岳耀敬[1,2] 郭婷婷[2] 王天翔 史兆国[1] 袁超[2] 王喜军 刘继刚 刘建斌[2] 张玲玲[2] 郭健[2] 牛春娥[2] 孙晓萍[2] 冯瑞林[2] 李范文 杨博辉[1,2] 

机构地区：[1]甘肃农业大学动物科学技术学院,甘肃兰州730070 [2]中国农业科学院羊育种工程中心,中国农业科学院兰州畜牧与兽药研究所,甘肃兰州730050 [3]甘肃省绵羊繁育技术推广站,甘肃张掖734031

出　　处：《甘肃农业大学学报》2016年第5期1-6,共6页Journal of Gansu Agricultural University

基　　金：国家绒毛用羊产业技术体系“分子育种岗位”(Nycytx-40-3);中国农业科学院“细毛羊资源与育种”创新工程(CAAS-ASTIP-2015-LIHPS);国家自然科学青年基金“基于单细胞测序研究非编码RNA调控绵羊次级毛囊发生的分子机制”(31402057);中央级公益性科研院所基本科研业务费专项资金项目(BRF1610322014006)

摘　　要：【目的】研究P-cadherin在‘高山美利奴羊’皮肤毛囊(hair follicle,HF)基板形成过程中的分布及表达情况,初步探讨P-cadherin是否可以作为‘高山美利奴’羊毛囊基板的标记物.【方法】以90-114d胎龄的‘高山美利奴羊’腹部皮肤作为材料,通过冰冻切片、HE染色、荧光定量PCR、免疫组化技术研究P-cadherin在毛囊基板形成过程中的表达规律.【结果】免疫组化结果显示,P-cadherin在胚胎毛囊形成时期的表皮、表皮基底层、真皮、隆突基底层、基板以及毛芽中都呈现阳性表达.荧光定量结果显示,在胎龄90、96、102、108、114d的相对表达量分别为(0.016 7±0.008 4)(0.113 4±0.052 9)(0.937 8±0.245 3)(0.068 4±0.026 8)(0.062 3±0.045 6).90d与96d相比差异显著(P=0.049 3<0.05),96d与102d相比差异不显著(P=0.088 9>0.05),102d与108d相比差异显著(P=0.023 5<0.05),108d与114d相比差异不显著(P=0.512 4>0.05).96d以后的相对表达量高于90d的相对表达量.90d到102d相对表达量逐渐升高,102d以后相对表达量逐渐降低,108d以后相对表达量趋于平稳,但是略高于114d的相对表达量,有降低的趋势.【结论】初步可以断定P-cadherin可以作为‘高山美利奴羊’毛囊基板的标记物.【Objective】To analyze the expression of P-cadherin on Alpine Merino sheep skin during hair follicle（HF）placode morphogenesis,and to develop a marker of hair follicle placode.【Method】GansuAlpine sheep skin from 90 dto 114dgestational age of on the abdomen was used as material,through the frozen sections,hematoxylin and eosin（HE）staining,fluorescence quantitative PCR and immunohistochemistry of technology to research the expression of P-cadherin during hair follicle placode morphogenesis.【Result】P-cadherin was expressed in the epidermis,the basal layer of the epidermis,dermis,carina basal layer,placode and hair buds during hair follicle placode morphogenesis.The relative expression levels of 90 d,96d,102,108 d,114dwere（0.008 4±0.016 7）（0.113 4±0.052 9）（0.937 8±0.245 3）（0.068 4±0.026 8）（0.062 3±0.045 6）,respectively.There was a significantly different between 90 dand 96d（P=0.049 30.05）,and 102 dand 108d（P=0.023 50.05）.No significant was found between 96dand102d（P=0.088 90.05）,and 108 dand 114d（P=0.512 40.05）.After 90 d,the relative expression level gradually increased,the relative expression level of 102 dreached the maximum,and the relative expression amount decreased gradually after 102 d,and the relative expression level was stable after 108 d.【Conclusion】P-cadherin can be used as a marker of Alpine Merino sheep hair follicle placode.

关 键 词：甘肃'高山美利奴羊’ 毛囊基板 P-CADHERIN 

分 类 号：S826.91[农业科学—畜牧学]