‘荷斯坦奶牛’产后期乳汁差异蛋白质组学研究  

Differential proteomic analysis of milk in the postpartum period of Holstein cows

作　　者：刘姗姗[1] 陶金忠[2] 赵国顺[3] 赵兴绪[4] 杨永新 

机构地区：[1]甘肃农业大学生命科学技术学院,甘肃兰州730070 [2]宁夏大学农学院,宁夏银川750021 [3]天水市动物疫病预防控制中心,甘肃天水741000 [4]甘肃农业大学动物医学院,甘肃兰州730070 [5]安徽农科院畜牧兽医研究所,安徽合肥230031

出　　处：《甘肃农业大学学报》2016年第5期7-13,共7页Journal of Gansu Agricultural University

基　　金：国家自然科学基金项目(31160523)

摘　　要：【目的】为了比较‘荷斯坦奶牛’产后期乳汁中蛋白质组表达的差异.【方法】应用双向电泳(2-DE)技术构建荷斯坦奶牛分娩当天,分娩后7d,分娩后15d,分娩后28d和分娩后35d乳汁二维凝胶电泳图谱,凝胶经考马斯亮蓝染色后,以3倍以上的表达变化用PDQuest 8.0分析.【结果】在这5个不同的时期共发现了8种差异蛋白,经MADIL-TOF-TOF串联质谱法分析发现这8种蛋白分别是:α-S2酪蛋白前体、β-酪蛋白、κ-酪蛋白前体、免疫球蛋白重链前体、白蛋白、血清白蛋白前体、cGMP磷酸二酯酶的α亚基、角蛋白I型细胞骨架10.【结论】根据这几种蛋白质的已有文献研究和蛋白含量在不同时期的变化趋势,表明这些蛋白可能与初乳的被动免疫、物质运输和泌乳有关.【Objective】To compare the difference in proteome expression of milk among Holstein cows in the postpartum period.【Method】The map of 0,7,15,28,35 days after delivery were performed using two-dimensional gel electrophoresis（2-DE）method.Differential protein spots were detected by PDQuest8.0software after staining with commassie blue.【Result】These spots were identified by MALDI-TOF/TOF-MS and eight differential proteins were found,which were alpha-s2-like casein precursor,beta-casein,kappa-casein precursor,immunoglobulin heavy chain precursor（B/MT.4A.17.H5.A5）-bovine,albumin,serum albumin precursor,cGMP phosphodiesterase alpha subunit and keratin I cytoskeletal 10.【Conclusion】According to reference research on the proteins and the changing trend of protein content in different period,these proteins may be related to the passive immunization of colostrums,material transport and lactation.

关 键 词：荷斯坦奶牛 产后期 乳汁 蛋白质组 

分 类 号：S823.91[农业科学—畜牧学]