多元模块工程在代谢工程中的应用与研究进展  

Progress and application on multivariate modular metabolic engineering in metabolic engineering

作　　者：刘丁玉[1,2,3] 孟娇[1,2,3] 王智文[1,2,3] 陈涛[1,2,3] 赵学明[1,2,3] 

机构地区：[1]天津大学化工学院,天津300072 [2]教育部系统生物工程重点实验室,天津300072 [3]天津化学化工协同创新中心,天津300072

出　　处：《化工进展》2016年第11期3619-3626,共8页Chemical Industry and Engineering Progress

基　　金：国家自然科学基金(NSFC-21576200;NSFC-21576191);国家973计划(2012CB725203);天津市自然科学基金(15JCQNJC06000)项目

摘　　要：随着代谢工程理论体系的发展,代谢工程的研究方法目前已从对单一途径的调控转变为对整个代谢网络的全局调控。同时,为了在工业微生物领域实现与化学工业生产规模相当的生物炼制过程,代谢工程需要一套通用的菌株优化策略。其中关键问题之一,是解决代谢通量的不平衡。本文介绍了基于传统的理性代谢工程与近年来兴起的组合工程中存在的问题,研究者提出了一种模块化的代谢网络优化策略——多元模块工程(multivariate modular metabolic engineering,MMME)。阐述了多元模块工程的原理和方法,列举了其常用的调控技术和手段,在此基础上综述了近年来模块化策略在代谢工程领域的应用进展,提出了该策略面临的主要问题并展望了其未来的发展方向。With the development of metabolic engineering,the metabolic engineering research method of single pathway regulation has evolved into global metabolic network regulation. In order to realize the chemical industrial vision of ‘biorefineries＇ in the field of industrial biotechnology,metabolic engineering requires a systematic study with well-defined principles and tools. One of the key problems is balancing metabolic flux. Based on the traditional,rational metabolic engineering and the rise of the problems existing in the combinatorial engineering in recent years,the researchers proposed a modular metabolic network optimization strategy using multiple module projects. The modular strategies in progress of application in metabolic engineering during recent years are summarized. In addition,the main problem and future direction of this strategy to optimize metabolic pathways are presented in light of the current knowledge of multiple module application.

关 键 词：多元模块工程 代谢工程 组合工程 合成生物学 

分 类 号：Q819[生物学—生物工程]