黄瓜第1雌花节位和雌花率基因QTL定位  

QTL Mapping of the First Female Flower Node and Female Flower Ratio in Cucumber(Cucumis sativus L.)

作　　者：曲美玲[1] 朱文莹[1] 杜慧[1] 潘俊松[1] 何欢乐[1] 蔡润[1] 

机构地区：[1]上海交通大学农业与生物学院,上海200240

出　　处：《上海交通大学学报（农业科学版）》2016年第5期8-16,共9页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：国家973项目(2012CB113900);国家自然科学基金项目(31272185)

摘　　要：本研究利用以全雌黄瓜品系S1000和强雄黄瓜品系S1002为亲本的含153个单株的F2群体构建的高密度遗传连锁图谱,通过调查F2:3群体第1雌花节位(first female flower node,FFFN)和雌花率(female flower ratio,SEX),对这2个性状进行了初步的QTL定位,并进一步对主效QTL所在区段进行候选基因分析。结果显示,第1雌花节位检测出1个QTL,位于第6染色体,为FFFN6.1,其贡献率(R^2)为9.39%;雌花率检测出2个QTL,位于第3和第6染色体,分别为SEX3.1(R^2为6.55%)和SEX6.1(R^2为14.03%)。推测FFFN6.1的候选基因为Csa6G216950,SEX6.1的候选基因很可能为F基因。In this study,based on a high-density genetic map constructed with the lines of gynoecious S1000,androecious S1002 and their 153F_2 individuals,we analyzed the QTLs of first female flower node and female flower ratio in cucumber.We made an investigation on the first female flower node and female flower ratio of F2：3groups and carried on the preliminary analysis.The results showed that one QTL of the first female flower node,FFFN6.1,was located on the Chr6,with 9.39% of the phenotypic variation explained（R^2）.Two QTLs related to the female flowers rate were detected,SEX3.1and SEX6.1,which are located on Chr3 and Chr6,with 6.55%and 14.03% of the phenotypic variation explained,respectively.By analyzing the candidate in their section of target traits QTL,we speculated that the candidate gene of FFFN6.1is Csa6G216950 and the candidate gene of SEX6.1may be the Fgene.

关 键 词：黄瓜 第1雌花节位 雌花率 QTLS 

分 类 号：S642.2[农业科学—蔬菜学]