低分子肝素对胃癌原代细胞增殖及SDF-1表达的影响  

Effects of Low Molecular Weight Heparin on the Proliferation and SDF-1 Expression of Primary Gastric Cancer Cells

作　　者：潘畅[1] 王书青[1] 邱明科 漆先琴 欧敬民[1] 沈军[1] 

机构地区：[1]上海交通大学医学院附属新华医院普外科,上海200092 [2]同济大学医学院附属上海第十人民医院普外科,上海200072

出　　处：《医学临床研究》2016年第10期1976-1979,共4页Journal of Clinical Research

摘　　要：[目的]探讨低分子肝素对胃癌原代培养细胞增殖及基质细胞衍生因子1（stromal cell derived factor-1，SDF-1）表达的影响。[方法]将胃低分化腺癌组织和正常胃黏膜组织制成原代细胞后分为：①胃癌细胞组（A组），②胃癌细胞＋低分子肝素（LMWH）组（B组），③胃黏膜细胞组（C组），④胃黏膜细胞＋LMWH组（D组）。MTT法检测各组细胞增殖，AnnexinV-FITC／PI双染法流式细胞术检测细胞周期分布和凋亡状况，RT-PCR检测各组细胞SDF-1mRNA的表达量并比较。[结果]B组细胞增殖显著低于A组，同时细胞凋亡率增加，G0和G1期细胞比例显著增高，而S期比例则显著减低，G2和M期无显著差异；D组细胞数量和细胞凋亡率、G0和G1期、S和G2和M期的比例与C组比较差异元统计学意义。B组与A组相比，SDF-1mRNA表达水平下降，而D组SDF-1mRNA表达水平与C组无明显差异。[结论]LMWH可显著抑制胃癌原代细胞的增殖和增加细胞的凋亡率，影响细胞周期，同时可降低细胞SDF-1表达。[Objective]To investigate the effect of low molecular weight heparin （LMWH） on the proliferation and the SDF-lexpression of primary cultured gastric carcinoma cells. [Methods]Poorly differentiated adenocarcinoma of the stomach tissues and normal gastric mucosa tissues were made into primary ceils and then divided into four groups ：group A had gastric cancer cells, group B had gastric cancer cells ＋ LMWH, group C had gastric mucosal cells, and group D had gastric mucosal cells ＋ LMWH. The cell proliferation was detected by MTT assay, the cell cycle distribution and apoptosis was detected by V-FITC/PI assay, and the expression of SDF-1 mRNA was examined by semi-quantitative RT-PCR.[ Results] The proliferation of gastric carcinoma cells in group B was much lower than in group A. At the same time, cell apoptosis was higher in group B than in group A. In the GO and G1 phases,there was a higher ratio of cells but a lower ratio was observed in the S period. There was no significant difference in cell ratios in the G2 and M phases between these two groups.For groups C and D, there was no significant difference for cell proliferation, cell cycle, or apoptosis. The expression of SDF-1 mRNA was lower in group B than in group A. There was no significant difference in the expression of SDF-1 mRNA in groups C and D. [Conclusion]LMWH can inhibit the proliferation of primary gastric carcinoma cells and also increase the cell apoptosis, changing the structure of the cell cycle and reducing the expression of SDF-1 mRNA in primary cells of gastric cancer.

关 键 词：胃肿瘤/病理学 肿瘤细胞 培养的 细胞增殖 肝素 低分子量 趋化因子CXCL12 

分 类 号：R457.7[医药卫生—治疗学]