B淋巴细胞微粒在类风湿关节炎患者外周血和关节液中的表达及临床意义  

Association of elevated B-lymphocyte Microparticles with disease activity in rheumatoid arthritis

作　　者：薛丽佳[1] 黄巧容[2] 林辉[1] 崔贝贝[1] 李茜 刘毅[1] 

机构地区：[1]四川大学华西医院风湿免疫科,四川成都610041 [2]四川大学华西医院干细胞生物学研究室,四川成都610041 [3]西南医科大学附属医院风湿免疫科,四川泸州646000

出　　处：《西部医学》2016年第11期1502-1505,共4页Medical Journal of West China

基　　金：国家自然科学基金(81102274);四川省科技厅国际合作项目(2014HH0027);成都市科技攻关项目(10GGYB644SF-023)

摘　　要：目的探讨B淋巴细胞微粒(B-LMPs)在类风湿关节炎(RA)发病机制中的作用。方法采用流式细胞术检测39例活动期RA患者(RA组)和24例健康体检者(健康对照组)外周血B-LMPs含量,检测RA患者外周血CRP、ESR、RF、ACPA指标,分析B-LMPs含量与各指标相关性。其中20例有膝关节积液RA患者(关节液组)同时检测配对外周血和关节液B-LMPs含量。结果 RA患者外周血B-LMPs含量显著高于健康对照组(P<0.01);RA患者关节液B-LMPs含量明显高于配对外周血(P<0.05)。RA患者外周血B-LMPs含量与疾病活动性评分DAS28呈正相关(r=0.5431,P=0.0009),与ESR、CRP、RF、ACPA无相关性;而关节液B-LMPs含量与疾病活动性评分DAS28呈负相关(r=-0.470,P=0.0362),与CRP、ESR、RF、ACPA无相关性。结论 RA患者外周血B-LMPs表达异常,且与疾病活动性显著相关,提示B-LMPs在RA疾病活动和关节损害中发挥重要作用。Objective To test the expression of B-lymphocyte Microparticles （B-LMPs） in rheumatoid arthritis （RA） patient＇s plasma and synovial fluid and compare the difference between patients and healthy controls （HC）. Methods The levels of B-LMPs in plasma were detected by flow eytometry in 39 active RA patients and 24 healthy volunteers. Synovial fluid （SF） was collected from 20 patients. Clinical parameters including CRP, ESR, RF, ACPA in the patients and volunteers were observed and analyzed. The potential correlation of B-LMPs counts and clinical parameters was analyzed. Results The B-LMPs levels in SF from RA patients were higher than paired plasma （P〈0.05） and in plasma from HC （P〈0.01）. The levels of plasma B-LMPs were positively correlated with Disease Activity Score DAS28 （r=0. 5431, P=0. 0009） and not correlated with ESR, CRP, RF and ACPA. B-LMPs levels in SF were not correlated DAS28, ESR, CRP, RF and ACPA （P〉0.05）. Conclusion B-LMPs may be involved in immune regulation and systemic inflammation of RA. The elevated levels of PMPs could be a potential biomarker for RA.

关 键 词：类风湿关节炎 B淋巴细胞微粒 流式细胞技术 关节液 

分 类 号：R593.22[医药卫生—内科学]