阿维菌素对半闭弯尾姬蜂保护酶系和解毒酶系的影响  

Effect of sublethal concentrations of abamectin on protective and detoxifying enzymes in Diagegma semclausum

作　　者：贾变桃[1] 洪珊珊[1] 张雨超[1] 曹永伟[1] 

机构地区：[1]山西农业大学农学院,山西太谷030801

出　　处：《环境昆虫学报》2016年第5期990-995,共6页Journal of Environmental Entomology

基　　金：山西省自然科学基金项目(2012011035-3);山西农业大学引进人才科研启动基金项目(XB2007008);山西省研究生创新项目(20143091);山西省大学生创新项目(2014097)

摘　　要：为明确阿维菌素对半闭弯尾姬蜂保护酶系和解毒酶系的影响,采用试管药膜法以阿维菌素亚致死浓度(LC10和LC25)处理半闭弯尾姬蜂成虫,分别于处理后1、12、24和48 h测定其体内保护酶和解毒酶活性变化。结果表明,阿维菌素亚致死浓度处理后,随着时间的延长,对寄生蜂体内超氧化物歧化酶(SOD)和过氧化物酶(POD)活性总体表现为诱导作用;对过氧化氢酶(CAT)活性表现为先抑制后诱导作用;对酯酶(EST)、谷胱甘肽S-转移酶(GST)和多功能氧化酶(MFO)具有显著的抑制作用。研究结果为深入了解半闭弯尾姬蜂对阿维菌素的防御机理提供了一定的理论基础。To explore the effect of abamectin on protective and detoxifying enzymes of Diadegma semiclausum Hellen,a larval parasitoid of diamondback moth,the activities of protective and detoxifying enzymes were determined after treatment of D. semiclausum adults for 1,12,24 and 48 hours with LC10 and LC25concentrations of abamectin using residual film method. The results showed that the activities of superxidase dismutase（SOD） and peroxidase（POD） were significantly promoted with increasing treatment time and the catalase（CAT） activity was firstly inhibited then promoted by sublethal concentrations of abamectin. At the same time, abamectin exposure inhibited the activities of detoxifying enzymes significantly at most of treatment time. The results provide some theory basis for further understanding defense mechanisms of D. semiclausum against abamectin.

关 键 词：阿维菌素 半闭弯尾姬蜂 保护酶 解毒酶 

分 类 号：Q965[生物学—昆虫学] S476[农业科学—农业昆虫与害虫防治]