白菜型油菜RAV基因的克隆及表达分析  

Cloning and expression analysis of transcription factor RAV from Brasserie campestris

作　　者：张腾国[1] 李萍[1] 寇明刚 王娟[1] 郑晟[1] 

机构地区：[1]西北师范大学生命科学学院,甘肃兰州730070

出　　处：《西北师范大学学报（自然科学版）》2016年第6期85-91,102,共8页Journal of Northwest Normal University(Natural Science)

基　　金：国家自然科学基金资助项目(31460099;31160089)

摘　　要：基于拟南芥RAV类转录因子的序列,通过RACE方法获得白菜型油菜RAV基因的cDNA序列,全长1 306bp,其中包括5′-UTR109bp,3′-UTR171bp,开放阅读框1 026bp,编码341个氨基酸,预测蛋白分子量为38.03u,理论等电点为9.2,含有相对保守的AP2和B3结构域.多序列比对和系统进化分析表明,油菜RAV与拟南芥RAV1具有很高的一致性,为88.0%.实时荧光定量PCR结果表明,油菜RAV基因受低温和盐胁迫的诱导,推测该基因在响应非生物胁迫中发挥重要作用.The sequences of RAV gene from Brassica campestris is obtained by RACE method based on the RAV factor sequence of Arabidopsis thaliana ,the full-length cDNA of RAV is 1036 bp,containing a 5′-UTR of 109 bp,a 3′-UTR of 171 bp,and a 1026 bp opening reading frame.The deduced protein is 341 amino acids with molecular weight 38.03 u and isoelectric point 9.2.The transcription factor of RAV contains two distinct DNA domains,one AP2 domain together with one B3 domain.Comparison of amino acid sequences and phylogenetic analysis indicates that this gene has high identities with Arabidopsis thaliana （88.0%）. qRT-PCR results indicates that RAV is induced by cold and NaCl. It shows the expression of the RAV plays an important role during abiotic stress in Brasserie campestris .

关 键 词：油菜 RAV转录因子 克隆 表达分析 

分 类 号：Q945.78[生物学—植物学]