结合基因芯片和生物信息学工具构建D-半乳糖致衰老小鼠模型的分子调控网络  

Analyzing the molecular regulatory networks in aging mouse induced by D-galactose using microarray combined with the MAS tool

作　　者：王晶[1,2] 赵翊[1] 王勇[1] 陈彻[1] 李海龙[1,2] 

机构地区：[1]甘肃中医药大学医学技术学院,甘肃兰州730000 [2]甘肃中医药大学甘肃省中药新产品创制工程实验室,甘肃兰州730000

出　　处：《西北师范大学学报（自然科学版）》2016年第6期92-97,共6页Journal of Northwest Normal University(Natural Science)

基　　金：甘肃省高等学校科研项目(2013A-087);甘肃省财政厅项目(BH-2013-24);甘肃中医药大学中青年科研基金资助项目(ZQ2015-20)

摘　　要：为构建衰老模型的分子调控网络,本研究以D-半乳糖致衰老小鼠为模型,利用小鼠全基因组表达谱芯片和生物信息学工具分析模型小鼠肾脏组织的分子变化.衰老模型与对照组相比,小鼠肾小球体积增大,系膜细胞数量相对减少;差异表达的基因有232个(变化倍数≥2);GO和信号通路分析显示差异表达基因主要参与生物节律、药物代谢和PPAR信号通路等,其构成的分子调控网络,主要有四个核心调控分子,即Arnt1、Cidea、Hspa1b和Anxa13.这些基因将是研究衰老机制的潜在靶点,有助于抗衰老药物筛选及治疗研究.To explore the target genes related with aging and construct the molecular regulatory network in aging model,the aging mice induce by D-galactose are used.The genome-wide oligonucleotide microarray and MAS tool are used to analyze the network which the regulated genes are involved in aging model.The morphology of kidney of aging mice are abnormal adversely compared with normal mice.There are 232 significantly regulated genes （fold changes ≥ 2 ）in aging mice verse normal mice. These differentially expresse genes participate in many important biological processes, including circadian rhythm, metabolism,and PPAR signaling pathway. There are four core genes in the network related with differentially expresse genes, including Arnt 1, Cidea , H spa 1b , and Anxa 13, which are potential targets of aging mechanism,and useful for drug screening.

关 键 词：基因芯片 生物信息学 衰老模型 分子网络 

分 类 号：R339[医药卫生—人体生理学]