PAI-1基因4G/5G多态性与儿童哮喘的易感性分析  

The-675 4G/5G polymorphism in plasminogen activator inhibitor-1 gene associated with asthma in children

作　　者：柴萍[1] 管立学[1] 李海波[1] 褚锦锦 张丽丽[1] 盖林林 

机构地区：[1]潍坊市人民医院,山东潍坊261041 [2]潍坊医学院医学检验系纳米医学技术研究所,山东潍坊261041

出　　处：《中国优生与遗传杂志》2016年第9期25-27,共3页Chinese Journal of Birth Health & Heredity

基　　金：潍坊市科技发展计划项目(NO.200761)

摘　　要：目的探讨纤溶酶原激活剂抑制物-1（Plasminogen activator inhibitor-1,PAI-1）基因启动子区-675bp处4G/5G多态性与潍坊地区汉族儿童哮喘发病的易感性及其临床特点的关系。方法采用病例-对照研究方法,选取196例哮喘患儿（儿童哮喘组）和173例健康儿童（对照组）为研究对象,应用限制性片段长度多态性（PCR-RFLP）分析方法检测PAI-1基因4G/5G多态性。结果儿童哮喘组和对照组之间,PAI-1基因3种基因型（4G/4G、4G/5G、5G/5G）频率分布差异有统计学意义（P〈0.01）。4G/4G基因型频率（28.0%）显著高于对照组（14.4%;P〈0.01）;与5G/5G基因型比较,携带4G/4G型儿童发生哮喘的相对风险（odds ratios,OR）增加2.95倍（95%CI：1.58~5.50;P〈0.01）。携带4G等位基因儿童发生哮喘的OR是5G等位基因型个体的1.61倍（95%CI：1.20~2.15;P〈0.01）。儿童哮喘组PAI-1基因不同基因型之间Ig E、CRP、LYM、EOS、NEU检测结果比较,差异均无统计学意义（P〉0.05）。结论 PAI-1基因4G/5G多态性与潍坊地区儿童哮喘的易感性相关,4G/4G型为易感基因型;该位点多态性与儿童哮喘Ig E、CRP、LYM、EOS、NEU等指标无相关性。Objective：To investigate associations between the-675 4G/5G polymorphism of plasminogen activator inhibitor-1（PAI-1） and asthma susceptibility and clinical characteristics of asthma in Han children in Weifang China. Methods：A casecontrol study was performed. One hundred and ninety-six children with asthma（asthmatic children group）were recruited in this study and 173 healthy children were selected as controls. The genotype of PAI-1 4G/5G polymorphism was assessed by polymerase chain reaction-restrictive fragment length polymorphism（PCR-RFLP）. Miettinen test was used to estimate odds ratios（OR）and 95% confidence interval（CI）. Results：Between asthmatic children group and controls,the distribution of three genotypes（4G/4G,4G/5G and 5G/5G） of PAI-1 gene had significant difference（P0.01）. The percentage of 4G/4G genotype was significantly greater in asthma group（28.0%）than in controls（14.4%;P0.01）.The PAI-1 polymorphism was associated with an increased risk of children asthma,and odds ratio was 2.95（95% CI：1.58-5.50;P0.01）for 4G/4G genotype compared with 5G/5G genotype. The frequency of 4G allele was higher in children with asthma than that in controls,resulting in an OR 1.61 to develop children asthma for 4G allele compared with 5G allele（95% CI：1.20-2.15;P0.01）. There were significant differences in the levels of Ig E and C-reaction protein（CRP）,lymphocyte counts（LYM）,eosinophils（EOS%） and neutrophils（NEU%） between 4G/4G genotype and 4G/5G or 5G/5G genotype in asthmatic children group. Conclusions：These results support that the hypothesis linking the 4G/4G PAI-1 genotype with an increased risk of children asthma. But genotypes of PAI-1 gene are not associated with Ig E level,CRP level,LYM counts,EOS% and NEU%.

关 键 词：哮喘 纤溶酶原激活剂抑制物-1 基因 多态性 易感性 儿童 

分 类 号：R725.6[医药卫生—儿科]