男性年龄对胚胎染色体及其辅助生殖结局影响的研究  

Infl uence of male age on the chromosome abnormalities in embryos and pregnancy outcomes of IVF-ET

作　　者：孙宝刚[1] 房姣[1] 王丽霞[1] 曹井贺[1] 张宁[1] 粱鲁南[1] 杨爱军[1] 

机构地区：[1]济宁医学院附属医院,山东济宁272000

出　　处：《中国优生与遗传杂志》2016年第9期115-117,共3页Chinese Journal of Birth Health & Heredity

基　　金：山东省医药卫生科技发展项目(2015WS0410);济宁市科技发展项目[济科字(2015)57号-15];济宁医学院附属医院苗圃科研项目[JYFY-MP-2013-(ZD)-005]

摘　　要：目的探讨男性年龄对胚胎染色体及其辅助生殖结局影响。方法选择在济宁医学院附属医院生殖中心接受IVF（In vitro fertilisation）/ICSI（Intracytoplasmic sperm injection）治疗的男性精液标本120例,按照男方年龄分为3组。〈35岁组（n=42）、35~39岁组（n=40）、≥40（n=38）,同时通过荧光原位杂交技术对以上3组患者通过常规体外受精周期中受精的第3天的废弃胚胎进行胚胎染色体分析,并对以上三组观察男方年龄对IVF的受精率、卵裂率、优质胚胎率、着床率、妊娠率及流产率的影响。结果≥40岁组胚胎染色体完全正常率39.6%（19/48）小于〈35岁组50.0%（31/62）及35~39岁组胚胎染色体完全正常率48.3%（28/58）,差异有统计学意义（P〈0.05）,〈35岁组50.0%（31/62）与35~39岁组胚胎染色体完全正常率48.3%（28/58）差异无统计学意义（P〉0.05）。男方年龄≥40岁组的受精率、卵裂率、优质胚胎率、植入率、妊娠率与男方年龄〈35岁组、35~39岁组比较,差异无统计学意义（P〉0.05）。而男方年龄≥40岁组患者的流产率17.65%（3/17）与男方年龄〈35岁4.34%（1/23）及35~39岁组5.00（1/20）相比明显升高,有统计学差异（P〈0.05）。结论男性年龄对受精率、胚胎质量、植入率、妊娠率、没有明显的相关性。而男性年龄对IVF胚胎染色体以及流产率有一定影响。在生育过程中,男性年龄应引起关注。Objective：To study the influence of the male age on embryo chromosomal abnormality and the outcome of conventional IVF-ET. Methods：We collected 120 semen samples and divided them into three groups according to the males′age：35 yr（n=43）,35-39 yr（n=31）,and,≥40 yr（n=30）. Based on the WHO Laboratory Manual（5th ed）,we detected the seminal parameters and aneuploidy of and abnormalities in embryos derived from abandoned zygotes producedby conventional IVF by fluorescence with specific probes for chromosomes13,18,21,X and Y among the three groups. We observed the rates of fertilization,cleavage,good quality embryo,implantation,clinical pregnancy and abortion in different groups. Results：There were no statistically significant differences among the three groups on sperm quality The men of the≥40 yr39.6%（19/48）group showed a significantly higher percentage of normalities in embryos than those of the35 yr groups 50.0%（31/62）and 35-39 yr groups48.3%（28/58）.but there were no statistically significant differences between 35 yr group and 35-39 yr group in abnormalities in embryos,There were no significant differences in the rates of fertilization,cleavage,good quality embryo,implantation,clinical pregnancy in the 40 yr group17.65%（3/17） and those of the35 yr group 4.34%（1/23）and the 35-39 yr group 5.00（1/20）（P〉0. 05）,while the abortion rate was markedly increased and there were significant differences during ≥40 yr group and the group of 35 yr and 35-39 yr.（P0. 05）.Couclusiou：Male age is not related obviously with the rates of fertilization,good quality embryo,implantation,and pregnancy. but it is related with the abortion and embryo chromosomal abnormality in IVF-ET.

关 键 词：男性年龄 三色荧光原位杂交 非整倍体率 胚胎染色体异常 

分 类 号：R714.8[医药卫生—妇产科学]