苯磺酸氨氯地平抑制高糖诱导的H9C2细胞凋亡研究  

Amlodipine besylate for suppressing H9C2 cells apoptosis induced by high glucose

作　　者：徐小红[1] 阮骆阳[2] 田小华[1] 潘凤娟[1] 杨彩兰[1] 

机构地区：[1]广东省农垦中心医院儿科,广东湛江524002 [2]广东省农垦中心医院麻醉科,广东湛江524002

出　　处：《重庆医学》2016年第31期4324-4326,共3页Chongqing medicine

基　　金：广东省湛江市政资金科技专项竞争性分配项目(2015A01037)

摘　　要：目的利用细胞实验探讨高糖培养对H9C2细胞凋亡的影响,并探讨苯磺酸氨氯地平的保护作用。方法体外培养大鼠心肌细胞H9C2,分为5mmol/L糖培养组(G1)、25mmol/L糖培养组(G2)、50mmol/L糖培养组(G3)和25mmol/L糖培养组加钙离子通道抑制剂络活喜保护组(G2+N)、50mmol/L糖培养组加络活喜保护组(G3+N)5组,每组分设48h(a)、72h(b)培养两个亚组共10组。AnnexinV/PI结合流式细胞仪检测细胞凋亡率,荧光染色观察[Ca2+]i。结果细胞凋亡率随着高糖刺激时间和高糖浓度的增加而逐渐增高,加络活喜组细胞的凋亡率显著降低(P<0.05)。G2、G3组单细胞平均[Ca2+]i活性测定荧光值均较G1组升高(P<0.05);G2+N、G3+N组单细胞平均[Ca2+]i活性测定荧光值分别较G2、G3组降低(P<0.05)。各a、b亚组间单细胞平均[Ca2+]i活性测定荧光值差异无统计学意义(P>0.05)。结论高糖培养H9C2细胞可增加[Ca2+]i从而导致细胞凋亡,苯磺酸氨氯地平可抑制Ca2+内流从而抑制细胞凋亡。Objective To study the influence of high glucose concentration culture on H9C2 apoptosis by using the cell experiment and to investigate the protective effect of amlodipine besylate. Methods The in vitro cutured rat cardiomyocytes H9C2 were divided into 5 groups：5 mmol/L gugar culture group （G1）, 25 mmol/L sugar culture group（G2）, 50 mmol/L sugar culture group（G3） and 25 mmol/L sugar culture group plus calcium ion channel inhibitor amlodipine besylate protection group（G2＋ N） and 50 mmol/L sugar culture group plus amlodipine besylate protection group（G3＋N）. Each group was re-divided into the 48 h culture subgroup （a） and 72 h culture subgroup（b）, 10 groups in total. The cellular apoptosis rate was detected by AnnexinV/PI combined with flow cytometer. Ca^2＋ was observed by the fluorescence staining. Results The cellular apoptosis rate was gradually increased with the high sugar stimulation time increase and high sugar concentration increase,which in the adding amlodipine besylate group was significantly decreased（P〈0.05）. The mean fluorescence value of [Ca^2＋ ]i of single cell in the group G2 and G3 was increased compared with the group G1 （P〈0.05）. The mean fluorescence value of [Ca^2＋ ]i activity of single cell in the group G2＋ N and G3＋N was decreased compared with the group G2 and G3（P〈0.05）. But the mean fluorescence value of [Ca^2＋ ]i activity of single cell had no statistically significant difference between various subgroup a and b （P〉0.05）. Conclusion Higher glucose culture of H9C2 cells can increase[Ca^2＋ ]i,thus causes the cellular apoptosis,amlodipine besylate can inhibit the Ca^2＋ internal flow, thus inhibits the cellular apoptosis.

关 键 词：糖尿病 妊娠 心肌细胞肥大 苯磺酸氨氯地平 

分 类 号：R714.256[医药卫生—妇产科学]