反相高效液相色谱法测定对乙酰氨基酚片的含量  

Content Determination of Acetaminophen Tablets by RP-HPLC

作　　者：逯小萌 

机构地区：[1]河南省濮阳市食品药品检验检测中心,濮阳457000

出　　处：《海峡药学》2016年第10期56-58,共3页Strait Pharmaceutical Journal

摘　　要：目的建立对乙酰氨基酚片的反相高效液相色谱法。方法色谱柱为Waters-C18(4.6mm×250mm,5μm),流动相:甲醇-1%冰醋酸溶液(30∶70),流速为1.0m L·min^(-1),柱温为35℃,检测波长为246nm。结果对乙酰氨基酚进样量在0.0402~1.0055μg范围内与峰面积呈良好线性关系,r=0.9997,平均回收率为99.43%,RSD=0.31%(n=9)。结论该方法准确度、灵敏度及重复性均较好,可作为对乙酰氨基酚片的含量测定。OBJECTIVE To establish a RP-HPLC method for the determination of acetaminophen tablets. METHODS Column Waters-C18（ 4. 6mm × 250 mm,5μm）,mobile phase was methanol∶1% acetic acid solution（ 30 ∶ 70）,the flow rate was 1. 0m L · min^（-1）,the column temperature was 35℃,the detection wavelength was246 nm. RESULTS The sample presented good linear relationship in the range of 0. 0402 ~ 1. 0055μg,r =0. 9997. The average recovery rate was 99. 43%,RSD = 0. 31%（ n = 9）. CONCLUSION The method was accurate,sensitive and reproducible,and can be used as an assay for the determination of the content of acetaminophen tablets.

关 键 词：对乙酰氨基酚片 反相高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学] R971.1