Plantlet regeneration through somatic embryogenesis in Nordmann's fir (Abies nordmanniana)  

作　　者：Katarzyna Nawrot-Chorabik 

机构地区：[1]Department of Forest Pathology, Mycology and Tree Physiology, University of Agriculture in Krakow

出　　处：《Journal of Forestry Research》2016年第6期1219-1228,共10页林业研究（英文版）

基　　金：supported by research topic DS No.3414 of the Ministry of Science and Higher Education and funded from international corporation Vitroflora

摘　　要：I studied the influence of various combinations of auxin and cytokinin concentrations, and the increased content of zinc and enzymatic casein hydrolizate in SH medium on initiation and proliferation of embryogenic callus of Abies nordmanniana （Steven） Spach. Addition- ally, the effect of ABA, PEG-4000 and different wave- lengths on the maturation of somatic embryos was tested. The use of optimum composition of modified SH medium with BA, KIN and 2.4-D while simultaneously ensuring appropriate external conditions resulted in 15.5 % embryogenesis. Finally, satisfactory results of microprop- agation of A. nordmanniana by somatic embryogenesis were obtained providing seven lines of embryogenic callus with high proliferation capacity. Those lines gave properly developed seedlings in white LED light with a wavelength of 400-700 nm, preceded by eight-week vernalization treatment of the callus. This paper may provide a protocol by which all stages of somatic embryogenesis of A. nord- manniana can be carded out, including the preceding 24-h seed disinfection with NaOCl and PVP, which resulted in 100 % frequency of uninfected zygotic embryos that were capable of starting embryogenesis.I studied the influence of various combinations of auxin and cytokinin concentrations, and the increased content of zinc and enzymatic casein hydrolizate in SH medium on initiation and proliferation of embryogenic callus of Abies nordmanniana （Steven） Spach. Addition- ally, the effect of ABA, PEG-4000 and different wave- lengths on the maturation of somatic embryos was tested. The use of optimum composition of modified SH medium with BA, KIN and 2.4-D while simultaneously ensuring appropriate external conditions resulted in 15.5 % embryogenesis. Finally, satisfactory results of microprop- agation of A. nordmanniana by somatic embryogenesis were obtained providing seven lines of embryogenic callus with high proliferation capacity. Those lines gave properly developed seedlings in white LED light with a wavelength of 400-700 nm, preceded by eight-week vernalization treatment of the callus. This paper may provide a protocol by which all stages of somatic embryogenesis of A. nord- manniana can be carded out, including the preceding 24-h seed disinfection with NaOCl and PVP, which resulted in 100 % frequency of uninfected zygotic embryos that were capable of starting embryogenesis.

关 键 词：ABIES In vitro - LED light POLYVINYLPYRROLIDONE Somatic embryogenesis 

分 类 号：S791.14[农业科学—林木遗传育种]