In vitro germination and shoot proliferation of the threatened species Cinchona officinalis L(Rubiaceae).  

作　　者：Rosa Armijos-Gonzalez Cesar Perez-Ruiz 

机构地区：[1]Departamento de Ciencias Naturales,Universidad Tecnica Particular de Loja,CP 11,01 608 Loja,Ecuador [2]Departamento de Biotecnologia y Biologia Vegetal,E.T.S.Ingenieros Agronomos,Universidad Politecnica de Madrid,CP 7,28040 Madrid,Spain

出　　处：《Journal of Forestry Research》2016年第6期1229-1236,共8页林业研究（英文版）

基　　金：the project‘‘PROY_IECOLOGIA_0036’’financed by the third internal call for projects of the Universidad Tcnica Particular de Loja(UTPL)

摘　　要：Cinchona officinalis （Rubiaceae） is an endemic species of the Loja Valley in southern Ecuador with medicinal uses. Because of over-exploitation in the nine- teenth century and more recent disturbances to its ecosystem, C. officinalis populations are threatened. Currently, natural regeneration of the populations is low, despite its high plant regeneration and seed formation capacity. In the present study, an efficient protocol for germination, shoot proliferation and plantlets regeneration was developed for this species. Phenolic content and germination rate of C. officinalis seeds were compared with a control species, C. pubescens. Nodal segments from seedlings of C. officinalis were cultured on Gamborg medium supplemented with different combinations of plant growth regulators. Because the phenol content is high in C. officinalis, the phenolic should be removed with hydrogen peroxide or water washes to stimulate germination. Shoots and callus developed from nodal segments within 45 days using most of the tested combinations of plant growth regulators. The best rates of shoot proliferation, callus formation and adventitious buds were obtained in medium supplemented with 5.0 mg L^-1 6-benzyl-aminopurine and 3.0 mg L^-1 indole- 3-butyfic acid.Cinchona officinalis （Rubiaceae） is an endemic species of the Loja Valley in southern Ecuador with medicinal uses. Because of over-exploitation in the nine- teenth century and more recent disturbances to its ecosystem, C. officinalis populations are threatened. Currently, natural regeneration of the populations is low, despite its high plant regeneration and seed formation capacity. In the present study, an efficient protocol for germination, shoot proliferation and plantlets regeneration was developed for this species. Phenolic content and germination rate of C. officinalis seeds were compared with a control species, C. pubescens. Nodal segments from seedlings of C. officinalis were cultured on Gamborg medium supplemented with different combinations of plant growth regulators. Because the phenol content is high in C. officinalis, the phenolic should be removed with hydrogen peroxide or water washes to stimulate germination. Shoots and callus developed from nodal segments within 45 days using most of the tested combinations of plant growth regulators. The best rates of shoot proliferation, callus formation and adventitious buds were obtained in medium supplemented with 5.0 mg L^-1 6-benzyl-aminopurine and 3.0 mg L^-1 indole- 3-butyfic acid.

关 键 词：CALLUS Cinchona officinalis Germination PHENOLS RUBIACEAE SHOOT 

分 类 号：S[农业科学]