鞘内注射丹参酮ⅡA对CCI大鼠脊髓背角内质网应激蛋白GRP78表达的影响  

Effects of Intrathecal Injection of Tanshinone IIA on Expression of GRP78 in Spinal Dorsal Horn of Neuropathic Pain Rats

作　　者：徐玉英[1] 游言文[1] 任秀花[2] 张艳霞[3] 

机构地区：[1]河南中医药大学,河南郑州450046 [2]郑州大学,河南郑州450001 [3]河南省中医院,河南郑州450002

出　　处：《中医学报》2016年第10期1542-1545,共4页Acta Chinese Medicine

基　　金：河南中医学院博士科研基金项目(BSJJ2012-10)

摘　　要：目的:观察丹参酮ⅡA对坐骨神经慢性压迫大鼠脊髓背角内葡萄糖调节蛋白78(glucose-regulated protein 78,GRP78)的表达,探讨内质网应激与丹参酮ⅡA镇痛机制之间的关系。方法:30只SD雄性大鼠随机分为模型组(n=20)和假手术组(n=10)。模型组又分为实验组和生理盐水组(n=10)。实验组和生理盐水组分别在大鼠鞘内注射丹参酮ⅡA 20 mg·kg^(-1)和生理盐水0.1 m L,在手术当日及术后,每日1次,连续注射14d。检测各组大鼠在手术前及术后14天d的机械痛阈和热痛阈;术后第14天,免疫组织化学法检测大鼠脊髓背角内GRP78的表达。结果:与假手术组比较,生理盐水组大鼠的GRP78的表达增多,机械痛阈和热痛阈明显降低;与生理盐水组比较,实验组的脊髓背角内GRP78的表达下降,机械痛阈和热痛阈明显升高,差异均有明显统计学意义(P<0.05)。结论:坐骨神经慢性压迫模型大鼠鞘内注射丹参酮ⅡA后的镇痛作用可能与降低模型大鼠脊髓背角内GRP78的表达有关。Objective： To observe effects of intrathecal injection of Tanshinone IIA on expression of GRP78 in spinal dorsal horn of neuropathic pain rats,and to explore the relationship between endoplasmic reticulum stress and the analgesic mechanism of Tanshinone IIA. Methods： 30 adult SD rats were randomly divided into two groups： CCI group（ n = 20） and sham group（ n = 10）. CCI group was randomly and evenly divided into two groups： treatment group（ TSA group） and saline group（ n = 10）. Tanshinone IIA20 mg·kg^-1 and nature saline 0. 1 mL were intrathecally injected respectively from the day of operation to 14 d after operation daily in the CCI group. Before and after operation,mechanical nociceptive thresholds were assessed with paw withdrawal thresholds（ PWTs） to von Frey filament and the paw withdrawal thermal latency（ PWTL） was examinated. GRP78 was measured with immunohistochemical staining method in the spinal dorsal horn of groups on 14 d after operation. Results： Compared with the sham group,the PWTs and PWTL decreased,the expression of GRP78 increased significantly in the saline group in the spinal dorsal horn 14 days after operation. Compared with the saline group,the PWTs and PWTL increased,the expression of GRP78 decreased significantly in the TSA group in the spinal dorsal horn 14 days after operation（ P〈0. 05）. Conclusion： Intrathecal injection ofTanshinone IIA can analgesic effect on neuropathic pain rats,which may through decreasing the expression of GRP78 in the spinal dorsal horn.

关 键 词：丹参酮ⅡA 神经病理性疼痛 坐骨神经慢性压迫 内质网应激 葡萄糖调节蛋白78 大鼠 中药 

分 类 号：R285.5[医药卫生—中药学]