血清miR-96作为乙型肝炎病毒相关肝细胞癌诊断标志物研究  

Study of serum microRNA-96 as a biomarker for hepatitis B virus related hepatocellular carcinoma

作　　者：梁丽娟[1] 沈涌海 陈岳明[3] 洪宝建[1] 朱月霞[1] LIANG Li-juan SHEN Yong-hai CHEN Yue-ming HONG Bao-jian ZHU Yue-xia(Clinical Laboratory,People＇s Hospital of Zhejiang, Hangzhou , Zhejiang 310010, China)

机构地区：[1]浙江省人民医院检验科,浙江杭州310014 [2]杭州市妇产科医院检验科,浙江杭州310008 [3]杭州市第一人民医院检验科,浙江杭州310006

出　　处：《中国卫生检验杂志》2016年第20期2939-2941,2944,共4页Chinese Journal of Health Laboratory Technology

基　　金：浙江省卫生厅资助项目(2014KYB024);杭州市科技局资助项目(20140733Q14)

摘　　要：目的探讨血清microRNA-96(miR-96)能否成为满意的乙型肝炎病毒(HBV)相关的肝细胞癌(HCC)生物标志物。方法本实验总计纳入414例研究对象,包括肝细胞癌(HCC)组104例、肝硬化(LC)组90例、慢性乙型病毒性肝炎(CHB)组100例及健康对照组120例,检测血清miR-96表达量。以miR-16为内参,用2-ΔCt方法计算血清miR-96相对表达量。结果血清miR-96在HCC组表达量显著高于其他3组,LC组高于CHB组及健康对照组,差异均有统计学意义(P<0.05)。以血清miR-96表达量0.138为临界值,CHB中诊断HCC的AUC为0.803,敏感度和特异度分别为77.9%和75.3%;血清miR-96联合AFP的AUC提高至0.889,敏感度和特异度分别为83.6%和82.4%。血清miR-96水平与肿瘤大小、淋巴结转移及高TNM分期相关(P<0.05)。结论本研究表明血清miR-96可能是一个有价值的HBV相关HCC的独立生物标志物。Objective To explore whether serum microRNA- 96（ miR- 96） could be a satisfactory biomarker for hepatocellular carcinoma（ HCC） related to hepatitis B virus（ HBV）. Methods 414 participants were enrolled in this study,and they were divided into the HCC group（ n = 104）,liver cirrhosis（ LC） group（ n = 90）,chronic hepatitis B（ CHB） group（ n = 100） and healthy control group（ n = 120）. Serum miR- 96 was measured,the levels of which were calculated by the 2- ΔCtmethod with miR- 16 as the internal control. Results Serum miR- 96 levels in HCC patients were remarkably higher than in the other groups,and that in LC group was higher than that in the CHB group and healthy control group,with the difference statistically significant（ P 〈0. 01）. With expression level（ 0. 138） of serum mir- 96 as cut- off,the area under curve（ AUC） of CHB in the diagnosis of HCC was 0. 083,and the sensitivity and specificity were 77. 9% and 75. 3%,respectively. Furthermore,the AUC for combined miR- 96 and α- fetoprotein（ AFP） was 0. 889,with sensitivity and specificity of 83. 6% and 82. 4% respectively. High serum miR- 96 levels were associated with larger tumor size,higher prevalence of lymph node metastasis and higher TNM stage（ P 〈 0. 05）. Conclusion Our findings suggest that serum miR- 96 is a promising biomarker for HCC patients with chronic HBV infection.

关 键 词：肝细胞癌 肝硬化 慢性乙型病毒性肝炎 生物标志物 微小核糖核酸-96 

分 类 号：R512.6[医药卫生—内科学]