Bt水稻秸秆还田对赤子爱胜蚓生长发育和生殖的影响  

Effect of Bt rice straw returning in soil on the growth and reproduction of Eisenia fetida

作　　者：程苗苗[1,2,3] 舒迎花[1,2,3,4] 王建武[1,2,3] CHENG Miao-miao SHU Ying-hua WANG Jian-wu(Institute of Tropical and Sub- tropical Ecology, South China Agricultural University, Guangzhou 510642, China Ministry of Ag- riculture Key Laboratory of Ecological Agriculture, South China Agricultural University, Guangzhou 510642, China Key Laboratory of Agroecology and Rural Environment of Guangclong Regular Higher Education Institutions, South China Agricultural University, Guangzhou 510642, China Department of Crop Science and Technology, College of Agriculture, South China Agricultural Uni- versity, Guangzhou 510642, China)

机构地区：[1]华南农业大学热带亚热带生态研究所,广州510642 [2]华南农业大学农业部生态农业重点开放实验室,广州510642 [3]华南农业大学广东省高等学校农业生态与农村环境重点实验室,广州510642 [4]华南农业大学农学院作物科学技术系,广州510642

出　　处：《应用生态学报》2016年第11期3667-3674,共8页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金项目(31170506;31470574)资助~~

摘　　要：Bt蛋白能通过转Bt基因作物的秸秆还田进入土壤,进而可能会对土壤动物如蚯蚓的生长发育和生殖造成影响.为评估Bt水稻对赤子爱胜蚓的影响,本文模拟秸秆还田,在土壤中添加2.5%、5%、7.5%和10%Bt水稻(b2B138)及其同源水稻(安丰A)秸秆,分别在饲养赤子爱胜蚓7、15、30、45、60、75和90 d后观测蚯蚓的存活率、相对生长率和生殖情况,以及秸秆土壤混合物和蚯蚓体内的Cry1Ab蛋白含量.结果表明:较高还田量(7.5%和10%)Bt水稻秸秆处理对赤子爱胜蚓存活率有抑制作用;Bt水稻秸秆还田对赤子爱胜蚓的相对生长率没有不利影响;还田量为5%、7.5%和10%时,Bt水稻秸秆还田能促进蚯蚓的生殖.酶联免疫吸附测定法(ELISA)结果表明:在Bt水稻土壤混合物中,蚯蚓体内均能检测到Cry1Ab蛋白,且前者随着时间延长而显著减少.因此,还田量为2.5%和5%时,Bt水稻秸秆还田释放的Cry1Ab蛋白对赤子爱胜蚓的生长发育和生殖没有不利影响.Bacillus thuringiensis （Bt） protein can enter the soil through Bt crops straw returning to field, which may affect the growth and reproduction of soil animals, such as earthworms. Here, Bt rice （b2B138） and conventional rice （Anfeng A） straw were returned in soil to evaluate the impact of Bt rice on Eisenia fetida. Two varieties of rice straw were added into soil to breed E. fetida at the rates of 2.5%, 5%, 7.5% and 10%. The survival rate, relative growth rate, reproduction of earthworm, the CryiAb content in soil-straw mixture and earthworm were detected after 7, 15, 30, 45, 60, 75, 90 d. The results showed that Bt rice straw returning at higher concentrations （7.5% and 10%） inhibited the survival rate of E. fetida. Bt rice straw returning had no adverse effect on rela- tive growth rate （RGR） of E. fetida. Bt rice straw treatment improved the reproduction of earth- worms under 5%, 7.5% and 10% straw returning in soil. Enzyme-linked immunosorbent assay （ELISA） results indicated that immunoreactive CrylAb was detectable in soil-straw mixture and E. fetida from Bt rice treatments, and a strong decline was observed in soil-straw mixture with the in- crease of treated time. Therefore, CrylAb released from Bt rice straw returning at 2.5% and 5% concentration had no adverse effects on the growth and reproduction of E. fetida.

关 键 词：转基因作物 土壤动物 BT蛋白 生态风险评估 

分 类 号：S141.4[农业科学—肥料学] S154.5[农业科学—农业基础科学]