转GAFP基因棉花生态适合度及其对棉田昆虫群落的影响  

Ecological fitness of transgenic GAFP cotton and its effects on the field insect community

作　　者：雒珺瑜[1] 张帅[1] 朱香镇 吕丽敏[1] 王春义[1] 李春花[1] 张利娟[1] 王丽[1] 崔金杰[1] LUO Jun-yu ZHANG Shuai ZHU Xiang-zhen LU Li-min WANG Chun-yi LI Chun-hua ZHANG Li-juan WANG Li CUI Jin-jie(Institute of Cotton Research of Chinese Academy of Agri- culture Sciences/State Key Laboratory of Cotton Biology, Anyang 455000, Henan, Chin)

机构地区：[1]中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南安阳455000

出　　处：《应用生态学报》2016年第11期3675-3681,共7页Chinese Journal of Applied Ecology

基　　金：转基因生物新品种培育重大专项(2016ZX08011-002)资助~~

摘　　要：新型转基因棉花生态适合度及其对棉田昆虫群落的影响是转基因棉花环境安全评价的重点内容.本文选用新型转GAFP(Gastrodia anti-fungal protein)基因棉花及其非转基因亲本棉花为试验材料,于2013和2014年在河南安阳研究了田间棉花生态适合度及其对昆虫群落的影响.结果表明:转基因棉蕾期、花铃期和铃期叶片干物质量与其亲本无显著差异;苗期比叶面积显著低于亲本,蕾期、花铃期和铃期叶面积显著大于亲本;苗期株高显著低于亲本,蕾期、花铃期和铃期株高与亲本无显著差异;棉花生长的4个关键时期(苗期、蕾期、花铃期和铃期),转基因棉单株果枝数、蕾数和脱落数均与亲本无显著差异,而铃期单株大铃数显著低于亲本.棉铃虫发生的3个高峰期,转基因棉对棉铃虫和甜菜夜蛾无明显控制作用,且对棉田昆虫群落、害虫亚群落和天敌亚群落的昆虫个体总数、昆虫群落结构与组成、均匀性指数、多样性指数和优势集中性指数均无显著影响.表明外源基因GAFP导入后,新型转基因棉花营养生长增强,产量构成性状整体无显著变化,未引起棉田昆虫群落变化.研究初步明确了新型转基因棉花生存竞争势态和棉田昆虫群落发生规律与动态,可为新型转基因棉花环境安全评价提供理论依据,同时为转基因棉花环境安全评价积累科学数据.The ecological fitness of transgenic cotton and its cotton fields is one of the key aspects of the evaluation of the ton. New transgenic GAFP （Gastrodia anti-fungal protein） effects on the insect communities in environmental safety of transgenic cot- cotton and its parental varieties were used in this study to explore their ecological fitness and their effects on insect community infield in Anyang, Henan Province in 2013 and 2014. The results showed that there was no significant differ- ence in dry mass for transgenic cotton leaves compared to that of parental cotton. Specific leaf areas of transgenic cotton were lowered obviously at seedling stage, while enhanced significantly at bud- ding, flowering and bolling stages relative to parental cotton. The plant height of transgenic cotton was lowered only at seedling stage, and no significant difference was showed between the two cuhi- vars at budding, flowering and boiling stages. No significant differences were discovered on plant branch numbers, bud numbers and falling numbers between the transgenic cotton and control mate- rial in any of the four key stages during the cotton growth. However, the number of bolls per plant for transgenic cotton was lower than that of the control cotton at the bolling stage. In the 2nd, 3rd, and 4th generation of cotton bollworm （ Helicoverpa armigera）, the mortality rate of cotton bollworm and beet armyworm （Spodoptera exigua） of transgenic cotton had no significant difference with parental cotton. Compared to parental cotton, total individuals of insect community, pest sub-communities and enemy sub-communities in transgenic cotton field didn＇t show any significant difference. The above results showed that after the GAFP gene was imported into cotton, the cotton growth was enhanced significantly, while the whole yield component traits and the insect community in the field were not significantly changed. Our study on the competition of new transgenic cotton and survival of transgenic cotton insect communities in cott

关 键 词：转GAFP基因棉花 生态适合度 棉铃虫 甜菜夜蛾 昆虫群落结构与组成 生物多样性 

分 类 号：S435.62[农业科学—农业昆虫与害虫防治] S476[农业科学—植物保护]