新疆小麦TaLox-B1、TaLox-B2、TaLox-B3位点的等位变异及其分布规律  

Molecular Identification and Distribution of TaLox-B1,TaLox-B2and TaLox-B3 Loci in Xinjiang Wheat Cultivars

作　　者：白璐[1] 禹飞雄 茹仙古丽·牙生 吕文娟[2] 任毅[2] 谢磊[2] 耿洪伟[2] 

机构地区：[1]新疆农业大学科学技术学院,新疆乌鲁木齐830052 [2]新疆农业大学农学院,新疆农业大学生物技术重点实验室,新疆乌鲁木齐830052 [3]新疆巴州农业局,新疆库尔勒841000

出　　处：《麦类作物学报》2016年第11期1456-1463,共8页Journal of Triticeae Crops

基　　金：国家自然科学基金项目(31260327);新疆自治区级大学生创新项目(20150016);2014年度留学人员科技活动择优资助项目(耿洪伟)

摘　　要：面粉颜色是小麦品质分级的重要指标。为了给新疆小麦面粉白度改良提供依据,本研究利用位于4BS染色体上的功能标记LOX16、LOX18和Lox-B23,对123份新疆小麦品种(系)的TaLox-B1、TaLoxB2和TaLox-B3等位变异进行分子标记检测。结果表明,在123份新疆小麦品种(系)中,具有高LOX活性等位基因TaLox-B1a和低LOX活性等位基因TaLox-B1b的材料分别有33份(26.8%)和90份(73.2%);具有高LOX活性等位基因TaLox-B2a和低LOX活性等位基因TaLox-B2b的材料分别有122份(99.2%)和1份(0.8%);具有高LOX活性等位基因TaLox-B3a和低LOX活性等位基因TaLox-B3b的材料分别有95份(77.2%)和28份(22.8%)。在不同类型冬小麦品种(系)中,TaLox-B1a的分布频率表现为引进品种(系)>自育品种(系)>地方品种外,TaLox-B3a的分布频率表现为地方品种>引进品种(系)>自育品种(系);在不同类型春小麦中TaLox-B1a只存在于晚期品种,TaLox-B2a和TaLox-B3a的分布频率均表现为早期品种(100.0%)>晚期品种。所检测的新疆小麦品种(系)共有5种基因型组合,具有最高LOX活性的基因型组合TaLox-B1a/TaLox-B2a/TaLox-B3a和TaLox-B1b/TaLox-B2a/TaLox-B3a,具有中等LOX活性的基因型组合TaLox-B1b/TaLox-B2a/TaLox-B3b和TaLox-B1a/TaLox-B2a/TaLox-B3b以及具有低LOX活性的基因型组合TaLox-B1b/TaLox-B2b/TaLox-B3b的分布频率分别为23.6%、53.7%、19.5%、2.4%和0.8%。在新疆小麦资源中具有高LOX活性基因型组合的材料的频率高于全国平均水平。本研究将3个位点功能标记组合起来使用,能更有效地评价检测材料的LOX活性水平。The color of the wheat flour is an important index of wheat quality traits. This study used the functional markers LOX16,LOX18 and Lox-B23 ,based on the development of wheat 4BS chromo- some,and molecular markers were detected by TaLox-B1, TaLox-B2 and TaLox-B3 in 123 wheat vari- eties in Xinjiang. The results showed that,there were 33（26.8%）varieties with TaLox-Bla allele ex- hibited high Lox activity, 90（73.2%）varieties with TaLox-Blb allele showed low Lox activity; 122（99.2%） varieties with TaLox-B2a allele exhibited high Lox activity, only 1 （0. 8%）varieties with TaLox-B2b allele showed low Lox activity; 95（77.2%）varieties with TaLox-B2a allele exhibited high LOX activity,28（22.8%）varieties with TaLox-B2b allele showed low Lox activity. In different types of winter wheat varieties,the frequency distribution of TaLox-Bla was in order of introduced varieties 〉self-cultivated varieties 〉local varieties. In different types of spring wheat varieties, the TaLox-Bla exists only in the late varieties,and the distribution frequency of both TaLox-B2a and TaLox-B3a was in order of early varieties （100 %） 〉late varieties. The analysis of 3 loci genotype combinations indica- ted that there are 5 kinds of genetic combinations in Xinjiang wheat varieties,which were both TaLox- Bla/TaLox-B2a/TaLox-B3a and TaLox-Blb/TaLox-B2a/TaLox-B3a combinations exhibited the high- est LOX activity, and were both TaLox-Blb/TaLox-B2a/TaLox-B3b and TaLox-Bla/TaLox-B2a/ TaLox-B3b combinations exhibited high LOX activity, and TaLox-Blb/TaLox-B2b/TaLox-B3b showed the lowest LOX activity,and the distribution frequency was 23.6% ,53.7% ,19.5% ,2.4% and 0.8%, respectively. The genotype frequency of high LOX activity in wheat cultivars in Xinjiang was higher than the national average. In this study,3 loci functional markers were combined more effectively ap- plied to wheat quality molecular marker assisted selection.

关 键 词：新疆小麦 脂肪氧化酶 功能标记 变异组合 

分 类 号：S512.1[农业科学—作物学] S330