纳米金属氧化物对耐药基因水平转移的影响  

Horizontal transfer of drug resistance genes promoted by metal oxide nanoparticles

作　　者：丁诚实[1,2] 潘杰[1] 高园[3] 康美玲[2] 邱志刚[3] 李君文[3] DING Chengshi PAN Jie GAO Yuan KANG Meiling QIU Zhigang LI Junwen(College of Life Science, Shandong Normal University, Jinan 250014, China College of Life Sciences, Zaozhuang University, Zaozhuang 277160, China Institute of Health and Environmental Medicine, Academy of Military Medical Science, Tianjin 300050, China)

机构地区：[1]山东师范大学生命科学学院,山东济南250014 [2]枣庄学院生命科学学院,山东枣庄277160 [3]军事医学科学院卫生学环境医学研究所,天津300050

出　　处：《济南大学学报（自然科学版）》2016年第4期281-286,共6页Journal of University of Jinan(Science and Technology)

基　　金：国家自然科学基金(81202163);天津市应用基础与前沿技术研究计划(13JCYBJC19900)

摘　　要：为探讨纳米金属氧化物对耐药基因水平转移的影响,采用抗性筛选法研究不同尺寸、不同浓度金属氧化物对不同浓度、不同种类耐药基因水平转移入不同菌株的影响,电泳观察纳米材料与耐药基因的结合,荧光显微观察纳米材料转导耐药基因进入细胞的现象,荧光探针测定纳米材料对菌体活性氧自由基产生的影响;建立数学模型,分析不同转移方式在纳米金属氧化物促耐药基因水平转移作用中的贡献。结果表明:纳米金属氧化物特别是氧化铝能促进耐药基因以转化和转导方式转移,机制可能为氧化损伤;随着时间的延长,两种方式中转化的作用越来越大。To explore the horizontal transfer of drug resistance genes promoted by metal oxide nanoparticles, the effect of metal oxide nanoparticles of different sizes and concentration on the horizontal transfer of drug resistance genes of different kinds and concen- tration into different bacteria strains was studied by resistance selection. The combination of nanomateria and resistance genes was observed by gel electrophoresis. Resistance genes transducted by nanomaterials into bacterial cells and the oxidative damage mechanism were analyzed by fluorometric probes. The ratio change of different pathways was analyzed by a mathematical model. The results show that metal oxide nanoparticles, especially for nano-sized Al2O3 particles, can promote the horizontal transfer of drug resistance genes by transformation and transduction, and that the mechanism may be oxidative damage. As time goes by, the transformation becomes more and more important.

关 键 词：纳米金属氧化物 耐药基因 水平转移 氧化损伤 模型分析 

分 类 号：Q28[生物学—细胞生物学]