盐胁迫下盐爪爪耐盐候选基因表达量分析  

EXPRESSION ANALYSIS OF SALT-TOLERANT GENES IN KALIDIUM FOLIATUM UNDER SALT STRESS

作　　者：贾晋[1] 周媛[1] 蔡禄[1] 韩淼[1] 张明贺[1] JIA Jin ZHOU Yuan CAI Lu HAN Miao ZHANG Minghe(College of Life Science and Technology, Inner Mongolia University of Science and technology, Baotou 014010, China)

机构地区：[1]内蒙古科技大学生命科学与技术学院,包头014010

出　　处：《内蒙古农业大学学报（自然科学版）》2016年第3期67-71,共5页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：内蒙古自然科学基金"盐生植物盐爪爪磷脂氢谷胱甘肽过氧化物酶基因克隆及表达分析"(2015MS0336);内蒙古科技大学"李保卫大学生科技创新基金项目"(2014030)

摘　　要：以分布于内蒙古河套灌区重度盐渍土的盐生植物盐爪爪为材料,采用0mmol/L,100mmol/L,200mmol/L,300mmol/L,400mmol/L和500mmol/L Na Cl胁迫72h后提取盐爪爪总RNA,采用实时荧光定量PCR技术(q RT-PCR)分析盐胁迫下耐盐候选基因表达量。结果表明:Na Cl浓度低于200mmol/L时,核酮糖1,5-二磷酸羧化酶/加氧酶大亚基基因表达量随Na Cl浓度升高而上升,Na Cl为200mmol/L时表达量最高。ABC转运蛋白基因表达则受Na Cl抑制,表现为随Na Cl浓度上升表达量下降,Na Cl为400mmol/L时表达量最低。研究还发现,Na Cl浓度低于200mmol/L时,GRAS家族的SCL1转录因子、异胡豆苷合成酶及硫氧还蛋白基因表达水平较高,Na Cl浓度高于200mmol/L时,表达量则急剧下降。研究结果为盐爪爪耐盐候选基因筛选提供了依据,为耐盐基因功能验证奠定了基础。Halophyte Kalidium foliatum which distributed on salinized soil of Inner Mongolia was used as material in the research,the K. foliatum was stressed with Na Cl which of concentrations were 0,100,200,300,400 and 500 mmol / L（ 0mmol/L Na Cl treatment as control） for 72 hour,total RNA was extracted from K. foliatum,the specific primers were designed according to the EST sequence from our results,28 S r RNA was selected as the reference gene,expression of salt tolerance genes were analyzed by real- time quantitative PCR. The results showed ribosomal 1,5- two phosphate carboxylase / oxygenase expression was induced by low Na Cl when Na Cl was below 200 mmol / L,and reached a maximum when Na Cl was 200 mmol / L,while was inhibited by high Na Cl,ABC transport protein expression was sharply reduced with Na Cl increased,and was lowest when Na Cl was 400 mmol / L. The SCL1 transcription factor in GRAS family,Strictosidine synthase and Ferredoxin thioredoxin expression maintained a high level when Na Cl was lower than 200 mmol / L,then were sharply reduced when Na Cl was higher than200 mmol / L.

关 键 词：耐盐基因 差异表达 QRT-PCR 盐爪爪 

分 类 号：Q943.2[生物学—植物学]