厚藤Actin基因片段的克隆及序列分析  

Cloning and Sequence Analysis of Actin Gene Fragment from Ipomoea pes-caprae L

作　　者：郭艳[1,2] 张美[1] 夏快飞[1] 简曙光[1] 陈建通[1] GUO Yan ZHANG Mei XIA Kuaifei JIAN Shuguang CHEN Jiantong(South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, Guangdong, China University of Chinese Academy of Sciences, Beijing 100049, China)

机构地区：[1]中国科学院华南植物园,广东广州510650 [2]中国科学院大学,北京100049

出　　处：《氨基酸和生物资源》2016年第3期59-62,共4页Amino Acids & Biotic Resources

基　　金：中国科学院A类战略性先导科技专项(XDA13020500)

摘　　要：为研究功能基因在厚藤(Ipomoea pes-caprae L)中的表达和调控提供内参基因,本文根据Actin基因的保守区设计简并性引物,采用RT-PCR克隆厚藤的Actin基因片段,然后将获得的片段连接于克隆载体上进行测序,并运用分子生物学软件对该基因序列进行分析。结果表明,该基因片段大小为554bp,编码184个氨基酸;该序列与其他植物Actin基因的cDNA序列的同源性均在80%以上,与氨基酸序列的同源性在94%以上。由此得出,本研究克隆的基因序列为Actin基因片段,将其命名为IpActin1,并登录在GenBank,登录号为KU564627。To provide housekeeping genes for studying the expression and regulation of functional genes in Ipomoea pes-caprae L,we designed a pair of degenerated primers and cloned the partial ActincDNA by RT-PCR,according to the conservative regions of other plant Actins.The cDNA fragments were cloned into T vector and sequenced.The results showed that this DNA fragment,including 554 bp,encoded a partial Actin with 184 amino acids.Homology comparison with other Actinsequences showed that it shared over 80%nucleotide sequence identity and 94%amino acid sequence identity with other Actins.This cDNA is named IpActin1,and is registered into GenBank（accession number：KU564627）.

关 键 词：厚藤 ACTIN基因 克隆 序列分析 

分 类 号：Q943.2[生物学—植物学]