碳纳米管/石墨烯修饰电极的制备及在槲皮素检测中的应用  

Preparation of carbon nanotube/graphene modified electrode and its application in determination of quercetin

作　　者：陈红艳[1] 胡海洋[1] 胡伟武[2] 

机构地区：[1]北京林业大学理学院,北京100083 [2]中国地质大学(北京)水资源与环境学院,北京100083

出　　处：《中国农业大学学报》2016年第9期143-148,共6页Journal of China Agricultural University

基　　金：国家自然科学基金面上项目(31070490);中央高校基本科研业务费专项资金项目(2015ZCQ-LY-03)

摘　　要：为方便有效地检测黄酮类物质的含量,使用碳纳米管和石墨烯制备新型修饰电极,用铁氰化钾溶液作探针溶液考察电极的电化学性能,证明修饰后的电极具有较好的电活性。使用修饰电极结合循环伏安法和差分脉冲伏安法2种电化学方法对典型黄酮类化合物槲皮素进行分析检测。检测结果表明:使用该电极在对槲皮素进行检测可以表现出很好的响应性能。在pH为4.5的Brittion-Robinson(B-R)缓冲液中,槲皮素的差分脉冲测试峰电流值与其浓度于1×10^(-7)~1×10^(-5) mol/L存在较好的线性关系,线性回归方程为ipa=-1.447c-0.325,最低检出限为2×10^(-8) mol/L(S/N=3),修饰电极检测简便,灵敏度高,检出极限较低。应用该方法对造粒型苦荞茶中的槲皮素含量进行测定,结果表明:修饰电极在黄酮类化合物实际样品的检测分析中使用,测量结果可靠、准确,具备一定的实际应用价值。A new modified electrode was prepared by using carbon nanotube and grapheme to detect the content of flavonoids effectively and conveniently. The electrochemical performance of modified electrode was examined by using potassium ferricyanide solution and the result showed that modified it displayed better electrical activity. In the Brittion- Robinson buffer solution （pH 4.5）, the peak current of differential pulse voltammetry （DPV） for quercetin rose as its concentration increased,and a good linearity was observed over 1×10^-7-1×10^-5 mol/L of Brittion-Robinson buffer solution with a detection limit of 2×10^-8 mol/L （S/N = 3）. The Linear regression equation was ipa = - 1. 447c - 0. 325 （ipa： A,c： tool/L, r = 0. 993）. Modified electrode showed advantages such as simple detection,high sensitivity and low detection limit. By determining the quercetin content of buckwheat tea with this method,the reliability and accuracy of the new modified electrode it was proved successfully.

关 键 词：石墨烯 碳纳米管 修饰电极 差分脉冲伏安法 槲皮素 

分 类 号：O646.22[理学—物理化学]