落葵薯DNA条形码筛选及其近缘植物的分子鉴定  

DNA Barcode Screening and Identifying for Anredera cordifolia and Its Related Species

作　　者：杨丽莹[1] 苏荣坤[2] 蔡宇忆 叶永浩[1] 李书渊[1] YANG Li-ying SU Rong-kun CAI Yu-yi YE Yong-hao LI Shu-yuan(Guangdong Pharmaceutical University,Guangzhou 510006,China Hong Kong University,Hong Kong,China)

机构地区：[1]广东药科大学,广东广州510006 [2]香港大学

出　　处：《中药材》2016年第6期1236-1240,共5页Journal of Chinese Medicinal Materials

基　　金：项目基金:广东地方习用中药饮片质量标准研究(20134815026)

摘　　要：目的:利用DNA条形码鉴定落葵薯及其近缘植物。方法:对来自不同产地的28份落葵薯及其近缘植物的核基因ITS序列和ITS2序列、叶绿体基因matK序列、psbA-trnH序列和rbcL序列进行PCR扩增并测序,比较不同序列的PCR成功率及测序成功率,对各序列进行种内和种间变异分析,Barcoding gap检验,以及构建NJ树聚类分析,评估不同序列对落葵薯及其近缘植物的鉴别能力。结果:经PCR扩增、测序后发现落葵psbA-trnH序列出现碱基缺失事件,其他序列均扩增、测序成功;matK序列和rbcL序列的测序成功率均为100%,ITS序列和ITS2序列的测序成功率分别为78.75%和64.28%;4条序列中,ITS序列和matK序列的种内和种间距离分别在barcoding gap检验中明显分离;从NJ树来看,ITS序列、matK序列均可区分落葵薯及其近缘植物。结论:建议采用ITS序列及matK序列作为落葵薯及其近缘植物鉴定的DNA条形码。Objective： To identify Anredera cordifolia and its closely related species using the DNA barcode. Methods： 28 individuals of Anredera cordifolia and its close related species were collected from different habitats. ITS and ITS2 of ribosomal DNA,matK,rbcL and psb A-trn H of chloroplast DNA were amplified and sequenced. The amplification and sequencing success rate,barcoding gap,and NJ trees were used to evaluate the efficiency of species identification. Results： After amplified and sequenced,base deletion was occurred in psb A-trnH sequences of Basella alba. The sequencing success rates of mat K,rbc L,ITS and ITS2 were 100%,100%,78. 75% and64. 28%,respectively. Among the four DNA barcoding sequences,ITS and mat K had remarkable barcoding gap. The NJ tree showed that Anredera cordifolia could differed obviously from its closely related species by ITS and mat K. Conclusion： The sequences of ITS and matK provide an effective and fast tool for the identification and authentication of medicinal plant of Anredera cordifolia and its related species.

关 键 词：落葵薯 DNA条形码 ITS ITS2 MATK RBCL pbsA-trnH 

分 类 号：R282.5[医药卫生—中药学]